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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 570™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.
The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy5, and blocking of granulocyte activation3,4. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 301312).
Application References:
1. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. 2. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. 3. Rezzonico R, et al. 2001. Blood 97:2932. (Block) 4. Marsik C, et al. 2003. Shock 20:493. (Block) 5. David A, et al. 2003. J. Leukoc. Biol. 74:551. (IF) 6. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed 7. Thurlow LR, et al. 2010. Infect. Immun. 128:1128. PubMed 8. Jadhav S, et al. 2001. Blood 167:5986. (Block) 9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 10. Sestak K, et al. 2007. Vet. Immunol. Immunop. 119:21.
Human peripheral blood granulocytes were stained with CD11b (clone ICRF44) Brilliant Violet 570™ (filled histogram), or mouse IgG1, κ Brilliant Violet 570™ (open histogram).
CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and Factor X.
Other Names:
Integrin αM chain, C3biR, CR3, Mac-1, Mo1
Structure:
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 KD
Distribution:
Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD11b
Human peripheral blood granulocytes stained with ICRF44 APC
Biotin anti-human CD11b
Human peripheral blood granulocytes stained with biotinylated ICRF44, followed by SAV-PE
LEAF™ Purified anti-human CD11b
Human peripheral blood granulocytes stained with LEAF™ purified ICRF44, followed by anti-mouse IgGs FITC
PE anti-human CD11b
Human peripheral blood lymphocytes, monocytes and granulocytes stained with ICRF44 PE
PE/Cy5 anti-human CD11b
Human peripheral blood lymphocytes, monocytes, and granulocytes stained with ICRF44 PE/Cy5
Purified anti-human CD11b
Human peripheral blood granulocytes stained with purified ICRF44, followed by anti-mouse IgGs FITC
Pacific Blue™ anti-human CD11b
Human peripheral blood granulocytes stained with ICRF44 Pacific Blue™
Alexa Fluor® 488 anti-human CD11b
Human peripheral blood lymphocytes, monocytes, and granulocytes stained with ICRF44 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD11b
Human peripheral blood granulocytes stained with ICRF44 Alexa Fluor® 647
PE/Cy7 anti-human CD11b
Human peripheral blood monocytes stained with ICRF44 PE/Cy7
PerCP/Cy5.5 anti-human CD11b
Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with CD11b (clone ICRF44) PerCP/Cy5.5 (top) or mouse IgG1, κ PerCP/Cy5.5 isotype control (bottom).
Brilliant Violet 421™ anti-human CD11b
Human peripheral blood granulocytes were stained with CD11b (clone ICRF44) Brilliant Violet 421™ (filled histogram), or mouse IgG1, κ Brilliant Violet 421™ (open histogram).
Brilliant Violet 570™ anti-human CD11b
Human peripheral blood granulocytes were stained with CD11b (clone ICRF44) Brilliant Violet 570™ (filled histogram), or mouse IgG1, κ Brilliant Violet 570™ (open histogram).
