Each lot of this Streptavidin-Brilliant Violet 421™ is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, we recommend using 405226 at dilutions ≤0.015 µg in 100 µl staining volume per million cells. For applications requiring high concentration of streptavidin reagent such as tetramer labeling, we recommend using 405225. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Streptavidin-Brilliant Violet 421™ is useful as a second step reagent for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies.

Brilliant Violet 421™ is suitable for confocal microscopy with a 405 or 407 nm laser, but it may not be optimal for widefield microscopy applications, such as epi- or trans-illuminating systems that use either Xenon or Mercury arc lamps or derivatives of those halides due to lack of commercially available optimized filter options that maximize the spectral characteristics of BV421.