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Brilliant Violet 421™ Mouse IgG2b, κ Isotype Ctrl Antibody
Brilliant Violet 421™ Mouse IgG2b, κ Isotype Ctrl Antibody
400341 25 tests $150.00     

Product Details

Clone: MPC-11
Isotype: Mouse IgG2b, κ
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested

Recommended Usage:

Each lot of this mouse IgG2b, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

  Learn more about Brilliant Violet™

For a guide on how to use Brilliant Violet™ conjugates in flow cytometry, download our technical sheet: Brilliant Violet™ Considerations for Multicolor Flow Cytometry.
COA:
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Application
Notes:

The MPC-11 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofleorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 400324) as negative control.

Application
References:

1. Smed-Sörensen A, et al. 2008. Blood 111:5037. (FA) PubMed
2. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed




Description:

The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

GeneID: NA
Latest Publications: View the latest IgG2b, kappa Isotype Ctrl articles on HighwirePress.com
UniProt: View information about IgG2b, kappa Isotype Ctrl on UniProt.org
Keywords: Brilliant Violet 421™ Mouse IgG2b, κ Isotype Ctrl, MPC-11, Brilliant Violet 421™, Immunology, Antibodies
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