Antibodies
Login
Search   
Brilliant Violet 421™ Goat anti-rat IgG (minimal x-reactivity) Antibody
Brilliant Violet 421™ Goat anti-rat IgG (minimal x-reactivity) Antibody
405414 125 µl $185.00     
Product Details
Clone: Poly4054
Isotype: Goat Ig
Reactivity: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

IF - Quality tested
FC
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescence staining. For IF staining, the suggested dilution of this reagent is 1:20 - 1:50. It is recommended that the reagent be titrated for optimal performance for each application. 

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
  Learn more about Brilliant Violet™

For a guide on how to use Brilliant Violet™ conjugates in flow cytometry, download our technical sheet: Brilliant Violet™ Considerations for Multicolor Flow Cytometry.
COA:
Enter Lot#:   
Application
Notes:

This conjugated goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.

Brilliant Violet 421™ is suitable for confocal microscopy with a 405 or 407 nm laser, but it may not be optimal for widefield microscopy applications, such as epi- or trans-illuminating systems that use either Xenon or Mercury arc lamps or derivatives of those halides due to lack of commercially available optimized filter options that maximize the spectral characteristics of BV421.
293E cells were stained with
293E cells were stained with purified anti-Histone H3-Phospho (Ser28) antibody (clone HTA28) and labeled with BV421™ anti-rat IgG (clone Poly4054)(blue). Cytosol is indicated by Alexa Fluor® 488 anti-tubulin-alpha (clone 10D8)(green) and nuclei are stained by propidium iodide. HTA28 only stains mitotic cells (violet).


Compare all formats



Description:

Goat anti-rat IgG antibody reacts with the heavy chains of rat IgG and the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.
GeneID: 295279
Latest Publications: View the latest Rat IgG articles on HighwirePress.com
UniProt: View information about Rat IgG on UniProt.org
Keywords: Brilliant Violet 421™ Goat anti-rat IgG (minimal x-reactivity), Poly4054, Brilliant Violet 421™, Rat, Immunology, Antibodies
Related Products
DescriptionCloneApplications
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • PE/Cy7 Goat anti-rat IgG (minimal x-reactivity)
    C57BL/c mouse splenocytes stained with

    C57BL/c mouse splenocytes stained with purified rat anti-mouse CD45R/B220 (RA3-6B2), followed anti-rat IgG (Poly4054) PE/Cy7

  • DyLight™ 594 Goat anti-rat IgG (minimal x-reactivity)
    Immortalized murine bone marrow-derived macrophages

    Immortalized murine bone marrow-derived macrophages stimulated overnight with LPS were stained with Atto-488 phalloidin (green) and purified TNF-α (clone MP6-XT22), secondarily stained with Goat anti-Rat IgG Dylight 594 (red). Data provided by James Harris, Trinity College.

  • DyLight™ 405 Goat anti-rat IgG (minimal x-reactivity)
    Mitotic 293E cell was stained

    Mitotic 293E cell was stained with Purified anti-Histone H3-Phosphorylated (Ser28) Antibody (clone HTA28) and labeled with DyLight™ 405 anti-rat IgG (clone Poly4054 (blue)). Cytosol indicated by Alexa Fluor® 488 anti-Tubulin-alpha (clone 10D8 (green)) and nucleus by PI (red).

  • Brilliant Violet 421™ Goat anti-rat IgG (minimal x-reactivity)
    293E cells were stained with

    293E cells were stained with purified anti-Histone H3-Phospho (Ser28) antibody (clone HTA28) and labeled with BV421™ anti-rat IgG (clone Poly4054)(blue). Cytosol is indicated by Alexa Fluor® 488 anti-tubulin-alpha (clone 10D8)(green) and nuclei are stained by propidium iodide. HTA28 only stains mitotic cells (violet).

x
Copyright © 2012 BioLegend, Inc. All rights reserved.