Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

This Brilliant Violet 421™ conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry or microscopy.

Brilliant Violet 421™ is suitable for confocal microscopy with a 405 or 407 nm laser, but it may not be optimal for widefield microscopy applications, such as epi- or trans-illuminating systems that use either Xenon or Mercury arc lamps or derivatives of those halides due to lack of commercially available optimized filter options that maximize the spectral characteristics of BV421.