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Brilliant Violet 421™ anti-Tubulin-γ Antibody
Brilliant Violet 421™ anti-Tubulin-γ Antibody
629207 125 µl $185.00     
Product Details
Clone: 14C11
Isotype: Mouse IgG2b, κ
Reactivity: Human, Mouse, Rat
Immunogen: Peptide-KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

IF - Quality tested
Recommended Usage:

For immunofluorescent staining applications, a concentration of 1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
  Learn more about Brilliant Violet™

For a guide on how to use Brilliant Violet™ conjugates in flow cytometry, download our technical sheet: Brilliant Violet™ Considerations for Multicolor Flow Cytometry.
COA:
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Application
Notes:

The purified 14C11 antibody has been shown to be useful for Western blotting of human, mouse, and rat γ-tubulin.

Brilliant Violet 421™ is suitable for confocal microscopy with a 405 or 407 nm laser, but it may not be optimal for widefield microscopy applications, such as epi- or trans-illuminating systems that use either Xenon or Mercury arc lamps or derivatives of those halides due to lack of commercially available optimized filter options that maximize the spectral characteristics of BV421.



Description:

γ-tubulin is a 51 kD member of the tubulin superfamily and is conserved between yeast and mammals. This cytoskeletal protein is highly expressed in centrosomes and is thought to be involved in cytoskeletal organization and mitosis. γ-tubulin is expressed at highest levels during prophase to metaphase and can be modified by phosphorylation. This protein has been shown to interact with α-tubulin, paxillin, and RAC GTPase activated protein 1.
Other Names: γ-tubulin
Structure: Tubulin superfamily, highly conserved between yeast and mammals. Predicted molecular weight approximately 51 kD
Distribution: Cytoskeletal protein, high expressed in centrosomes
Function: Cytoskeletal organization, mitosis
Regulation: Expressed at highest levels during prophase to metaphase
Modification: Phosphorylation
Interaction: Interacts with α-tubulin, paxillin, RAC GTPase activated protein 1
Antigen
References:

1. Zheng Y, et al. 1991. Cell 65:817.
GeneID: 2776
Latest Publications: View the latest Tubulin-gamma articles on HighwirePress.com
UniProt: View information about Tubulin-gamma on UniProt.org
Keywords: Brilliant Violet 421™ anti-Tubulin-γ , 14C11, Brilliant Violet 421™, γ-tubulin, Human, Mouse, Rat, Immunology, Antibodies
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