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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Additional reported applications (for relevant formats) include: Western Blotting1.
This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. This availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
Application References:
1. Robbins SH, et al. 2002. J. Immunol. 168:2585. (WB) 2. Robbins SH, et al. 2003. J. Immunol. 170:5876. (FC) 3. McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)
C57BL/6 mouse splenocytes were stained with KLRG1 (clone 2F1/KLRG1) Brilliant Violet 421™ and NK1.1 APC.
Killer cell lectin-like receptor G1 (KLRG1) is the mouse homolog of the rat mast cell function-associated antigen (MAFA or 2F1-Ag). KLRG1 is a type II membrane glycoprotein that was first identified on the surface of rat mast cell line RBL-2H3. It is composed of a homodimer of glycosylated 30-38 kD subunits. Mouse and human homologs of KLRG1 are expressed by subsets of NK cells and lymphokine-activated killer (LAK) cells but not mast cells. KLRG1 is also expressed on subsets of CD8+ and CD4+ cells, including CD4+ and CD8+ effector/memory cells, potent regulatory CD4+ T cells. KLRG1 may be involved in regulating NK cell homeostasis. KLRG1 was recently found to recognize cadherins and thus inhibit immune responses by regulating the effector function and the developmental processes of NK and T cells.
Other Names:
MAFA, 2F1-Ag
Structure:
KLRG1 is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM, expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kD.
Distribution:
Subsets of NK cells, lymphokine-activated killer (LAK) cells and subsets of CD8+ and CD4+ cells.
Function:
Regulate NK cell proliferation, maturation and homeostasis. Inhibit immune responses by regulating the effector function and the developmental processes of NK and T cells.
Ligand Receptor:
E-cadherin
Antigen References:
1. Grundemann C, et al. 2006. J. Immunol. 176:1311. 2. Blaser C, et al. 1998. J. Immunol. 161:6451. 3. Huntington ND, et al. 2007. J. Immunol. 178:4764. 4. Voehringer D, et al. 2001. J. Immunol. 167:4834.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse KLRG1 (MAFA)
C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and purified 2F1/KLRG1 conjugated with PE
C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and Syrian hamster IgG PE isotype control
LEAF™ Purified anti-mouse KLRG1 (MAFA)
C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and purified 2F1/KLRG1 conjugated with PE
C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and Syrian hamster IgG PE isotype control
Biotin anti-mouse KLRG1 (MAFA)
C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and biotinylated 2F1/KLRG1, followed by Sav-PE
C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and biotinylated Syrian hamster IgG, followed by Sav-PE
PE anti-mouse KLRG1 (MAFA)
C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and 2F1/KLRG1 PE
C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and Syrian hamster IgG PE isotype control
APC anti-mouse KLRG1 (MAFA)
C57BL/6 mouse splenocytes stained with 2F1/KLRG1 APC and NK1.1 (PK136) PerCP
FITC anti-mouse KLRG1 (MAFA)
C57BL/6 mouse splenocytes stained with 2F1/KLRG1 FITC and NK1.1 (PK136) APC
Brilliant Violet 421™ anti-mouse KLRG1 (MAFA)
C57BL/6 mouse splenocytes were stained with KLRG1 (clone 2F1/KLRG1) Brilliant Violet 421™ and NK1.1 APC.
