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Brilliant Violet 421™ anti-mouse IgM Antibody
Brilliant Violet 421™ anti-mouse IgM Antibody
406517 125 µl $160.00     
Product Details
Clone: RMM-1
Isotype: Rat IgG2a, κ
Isotype Control:Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: mouse IgGs and IgM, IgE, IgA cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
  Learn more about Brilliant Violet™

For a guide on how to use Brilliant Violet™ conjugates in flow cytometry, download our technical sheet: Brilliant Violet™ Considerations for Multicolor Flow Cytometry.
COA:
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Application
Notes:

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Application
References:

1. Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
C57BL/6 splenocytes were stained with
C57BL/6 splenocytes were stained with CD19 FITC and anti-IgM (clone RMM-1) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ isotype control (bottom).




Compare all formats



Description:

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.
Other Names: Immunoglobulin M
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
GeneID: 16019
Latest Publications: View the latest IgM articles on HighwirePress.com
UniProt: View information about IgM on UniProt.org
Keywords: Brilliant Violet 421™ anti-mouse IgM, RMM-1, Brilliant Violet 421™, Immunoglobulin M, Mouse, Immunology, Antibodies
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DescriptionCloneApplications
Brilliant Violet 421™ Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Cell Staining BufferFC, ICC, ICFC
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TruStain fcX™ (anti-mouse CD16/32)93 FC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-mouse IgM
    C57BL/6 Mouse splenocytes stained with

    C57BL/6 Mouse splenocytes stained with purified RMM-1, followed by anti-rat IgG FITC

  • PE anti-mouse IgM
    C57BL/6 splenocytes stained with RMM-1

    C57BL/6 splenocytes stained with RMM-1 PE and 6D5 (CD19) APC

  • Biotin anti-mouse IgM
    C57BL/6 Mouse splenocytes stained with

    C57BL/6 Mouse splenocytes stained with biotinylated RMM-1, followed by Sav-PE

  • FITC anti-mouse IgM
    C57BL/6 splenocytes stained with RMM-1

    C57BL/6 splenocytes stained with RMM-1 FITC

  • APC anti-mouse IgM
    C57BL/6 splenocytes stained with RMM-1

    C57BL/6 splenocytes stained with RMM-1 APC

  • PerCP/Cy5.5 anti-mouse IgM
    C57BL/6 splenocytes stained with RMM-1

    C57BL/6 splenocytes stained with RMM-1 PerCP/Cy5.5

  • APC/Cy7 anti-mouse IgM
    C57BL/6 splenocytes stained with CD19

    C57BL/6 splenocytes stained with CD19 (6D5) FITC and RMM-1 APC/Cy7

    C57BL/6 splenocytes stained with CD19

    C57BL/6 splenocytes stained with CD19 (6D5) FITC and rat IgG2a APC/Cy7 isotype control

  • Brilliant Violet 421™ anti-mouse IgM
    C57BL/6 splenocytes were stained with

    C57BL/6 splenocytes were stained with CD19 FITC and anti-IgM (clone RMM-1) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ isotype control (bottom).





  • PE/Cy7 anti-mouse IgM
    C57BL/6 splenocytes stained with CD19

    C57BL/6 splenocytes stained with CD19 (6D5) APC and RMM-1 PE/Cy7

    C57BL/6 splenocytes stained with CD19

    C57BL/6 splenocytes stained with CD19 (6D5) APC and rat IgG2a PE/Cy7 isotype control

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