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Brilliant Violet 421™ anti-mouse CD335 (NKp46) Antibody
Brilliant Violet 421™ anti-mouse CD335 (NKp46) Antibody
137611 125 µl $185.00     
Product Details
Clone: 29A1.4
Isotype: Rat IgG2a, κ
Isotype Control:Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: NKP46-IgG1 Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
  Learn more about Brilliant Violet™

For a guide on how to use Brilliant Violet™ conjugates in flow cytometry, download our technical sheet: Brilliant Violet™ Considerations for Multicolor Flow Cytometry.
COA:
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Application
Notes:

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1,2 and in vitro activation of NK cells1.
Application
References:

1. Walzer T, et al. 2007. P. Natl. Acad. Sci. USA 104:3384. (IHC, Activ)
2. Walzer T, et al. 2007. Nat. Immunol. 8:1337. (IHC)
3. Guerriero JL, et al. 2011. J. Immunol. 186:3517. PubMed
C57BL/6 splenocytes stained with NK1.1
C57BL/6 splenocytes stained with NK1.1 APC and NKp46 (clone 29A1.4) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ isotype control (bottom).




Compare all formats



Description:

CD335, also known as NKp46, is a single-pass type I membrane protein of 46 kD. It belongs to the natural cytotoxicity receptor (NCR) family and contains two Ig-like (immunoglobulin-like) domains.

NKp46 expression is restricted to NK cells and a subset of NK-like T cells and is not expressed in CD1d-restricted NKT cells. NKp46 is a receptor for viral hemagglutinins and heparan sulfate proteoglycans and is involved in NK cell activation.
Other Names: NCR1
Structure: Single-pass type I membrane protein, 46 kD. Belongs to the natural cytotoxicity receptor (NCR) family. Contains 2 Ig-like (immunoglobulin-like) domains.
Distribution: Mature and immature NK cells, subset of NK-like T cells, but not on CD1d-restricted NKT cells
Function: NK cells activation
Ligand Receptor: Viral hemagglutinins, heparan sulfate proteoglycans
Antigen
References:

1. Colucci F and Cilio CM. 2010. Nat. Immunol. 125:60.
2. Caligiuri MA. 2008. Blood 112:461.
3. Colonna M. 2009. Immunity 31:15.
GeneID: 17086
Latest Publications: View the latest CD335 articles on HighwirePress.com
UniProt: View information about CD335 on UniProt.org
Keywords: Brilliant Violet 421™ anti-mouse CD335 (NKp46), 29A1.4, Brilliant Violet 421™, NCR1 , Mouse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • PE anti-mouse CD335 (NKp46)
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 29A1.4 PE and NK1.1 (PK136) APC

  • Purified anti-mouse CD335 (NKp46)
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with purified 29A1.4 conjugated with PE and NK1.1 (PK136) APC

  • FITC anti-mouse CD335 (NKp46)
    C57BL/6 splenocytes stained with NK1.1

    C57BL/6 splenocytes stained with NK1.1 (PK136) APC and 29A1.4 FITC

    C57BL/6 splenocytes stained with NK1.1

    C57BL/6 splenocytes stained with NK1.1 (PK136) APC and rat IgG2a, κ FITC isotype control

  • APC anti-mouse CD335 (NKp46)
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with NK1.1 (PK136) PE and 29A1.4 APC

    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with NK1.1 (PK136) PE and rat IgG2a APC isotype control

  • PerCP/Cy5.5 anti-mouse CD335 (NKp46)
    C57BL/6 splenocytes stained with NK-1.1

    C57BL/6 splenocytes stained with NK-1.1 (PK136) FITC and PerCP/Cy5.5 anti-mouse CD335 (clone 29A1.4)

    C57BL/6 splenocytes stained with NK-1.1

    C57BL/6 splenocytes stained with NK-1.1 (PK136) FITC and PerCP/Cy5.5 rat IgG2a isotype control

  • Brilliant Violet 421™ anti-mouse CD335 (NKp46)
    C57BL/6 splenocytes stained with NK1.1

    C57BL/6 splenocytes stained with NK1.1 APC and NKp46 (clone 29A1.4) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ isotype control (bottom).





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