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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining of acetone-fixed frozen sections1,4, in vitro stimulation of T cells1 and depletion of CD3+ cells in vivo2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 100208).
Application References:
1. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (IP, IHC, Activ, C') 2. Mysliwietz J, et al. 1992. Blood 80:2661. (Deplete) 3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (C') 4. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC) 5. Zan H, et al. 2005. EMBO J. 24:3757. 6. Morgado, P., et al. 2011. Infect Immun. 79:4401. PubMed 7. Xiao J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:386. PubMed
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Other Names:
T cell antigen receptor complex, T3
Structure:
Ig superfamily, CD3/TCR, 20 kD
Distribution:
Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function:
Antigen recognition, TCR signal transduction, T cell activation
Ligand Receptor:
Peptide antigen/MHC-complex
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse CD3
C57BL/6 splenocytes stained with 17A2 FITC and 145-2C11 PE
LEAF™ Purified anti-mouse CD3
C57BL/6 mouse splenocytes stained with LEAF™ purified 17A2, followed by anti-rat IgG FITC
PE anti-mouse CD3
C57BL/6 mouse splenocytes stained with 17A2 PE
Purified anti-mouse CD3
C57BL/6 mouse splenocytes stained with purified 17A2, followed by anti-rat IgG FITC
Alexa Fluor® 647 anti-mouse CD3
C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 647
Alexa Fluor® 488 anti-mouse CD3
C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 488
Pacific Blue™ anti-mouse CD3
C57BL/6 mouse splenocytes stained with Pacific Blue™ 17A2
Alexa Fluor® 700 anti-mouse CD3
C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 700
PerCP/Cy5.5 anti-mouse CD3
C57BL/6 splenocytes stained with 17A2 PerCP/Cy5.5
PE/Cy7 anti-mouse CD3
C57BL/6 splenocytes stained with 17A2 PE/Cy7
APC/Cy7 anti-mouse CD3
C57BL/6 splenocytes stained with 17A2 APC/Cy7 "
Brilliant Violet 421™ anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 570™ anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2 Brilliant Violet 570™ (top) or rat IgG2b, κ Brilliant Violet 570™ isotype control (bottom).
Brilliant Violet 650™ anti-mouse CD3
C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 650™.
