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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
1. Maier H, et al. 2007. J. Immunol. 178:2714. 2. Meng Q, et al. 2006. Invest. Ophth. Vis. Sci. 47:444. PubMed
C57BL/6 mouse splenocytes were stained with CD274 (clone 10F.9G2) Brilliant Violet 421™ (filled histogram), or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T lymphocyte proliferation and production of IL-10 and IFN-g, in an IL-2-dependent and a PDCD1-independent manner. Its interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Other Names:
B7-H1, PD-L1
Structure:
40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily
Distribution:
T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes
Ligand Receptor:
PD-1 (PDCD1)
Antigen References:
1. Sharpe A, et al. 2007. Nat. Immunol. 8:239. 2. Dong H, et al. 1999. Nat. Med. 5:1365. 3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD274 (B7-H1, PD-L1)
C57/B6 mouse splenocytes were stained with purified anti-CD274 (clone 10F.9G2) (green line) or purified rat IgG2b, κ PE/Cy7 (pink line).
LEAF™ Purified anti-mouse CD274 (B7-H1, PD-L1)
C57/B6 mouse splenocytes were stained with LEAF™ purified anti-CD274 (clone 10F.9G2) (green line) or purified rat IgG2b, κ (red line) followed by Sav-PE.
Biotin anti-mouse CD274 (B7-H1, PD-L1)
C57/B6 mouse splenocytes were stained with biotinylated anti-CD274 (clone 10F.9G2) (green line) or biotinylated rat IgG2b, κ (pink line) followed by Sav-PE.
PE anti-mouse CD274 (B7-H1, PD-L1)
C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) PE (filled histogram) or rat IgG2b, κ PE (open histogram).
C57BL/6 mouse splenocytes were stained with CD274 (clone 10F.9G2) Brilliant Violet 421™ (filled histogram), or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
APC anti-mouse CD274 (B7-H1, PD-L1)
C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) APC (pink line) or rat IgG2b, κ APC (purple line).
PE/Cy7 anti-mouse CD274 (B7-H1, PD-L1)
C57/B6 mouse splenocytes were stained with anti-CD274 (clone 10F.9G2) PE/Cy7 (orange line) or rat IgG2b, κ PE/Cy7 (purple line).
