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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. The mature CD107a is heavily glycosylated from a 40 kD core protein. It is expressed by macrophages, epithelial cells, endothelial cells, and some tumor cells. This molecule is located on the luminal side of lysosomes and has been suggested to play a role in the protection of the lysosome membrane from lysosomal hydrolases. CD107a is a ligand for galaptin. Upon activation, CD107a is transferred to the surface of the cell membrane. It plays a role in cell adhesion and regulation of tumor metastasis and mediates autoimmune disease progression.
Type I transmembrane glycoprotein, 40 kD core protein, mature protein is heavily glycosylated with 110-140 kD
Macrophages, epithelial cells, endothelial cells, some tumor cells, located on the luminal side of lysosomes or on the surface of cell membrane
Protect lysosomal membrane from lysosomal hydrolases, adhesion
1. Barclay AN, et al. 1997. The Leucocyte Antigen Facts Book. 2nd Edition. Academic Press. 2. Heffernan M, et al. 1989. Cancer Res. 49:6077. 3. Granger BL, et al. 1990. J. Bio. Chem. 265:12036. 4. De Carvalho Bittencourt M, et al. 2005. Eur. J. Immunol. 35:1501.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-mouse CD107a (LAMP-1)
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with 1D4B PE
Purified anti-mouse CD107a (LAMP-1)
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with purified 1D4B, followed by anti-rat IgG FITC
Biotin anti-mouse CD107a (LAMP-1)
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with biotinylated 1D4B, followed by Sav-PE
FITC anti-mouse CD107a (LAMP-1)
Thioglycollate-elicited BALB/c mouse peritoneal macrophages stained with 1D4B FITC