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Brilliant Violet 421™ anti-human TCR α/β Antibody
Brilliant Violet 421™ anti-human TCR α/β Antibody
306721 25 tests $185.00 Qty:
    
306722 100 tests $385.00 Qty:
    

Product Details

 
Clone: IP26 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

  Learn more about Brilliant Violet™

For a guide on how to use Brilliant Violet™ conjugates in flow cytometry, download our technical sheet: Brilliant Violet™ Considerations for Multicolor Flow Cytometry.
Excitation
Laser:
Violet Laser (405 nm)
COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone.  Other anti-CD3 clones may compete out the binding of IP26.  

Application
References:

  Publication Library
Brilliant Violet 421™ anti-human TCR α/β Antibody IP26 Image 1
Human peripheral blood lymphocytes were stained with anti-human TCRα/β (clone IP26) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).


Compare all formats

See Brilliant Violet 421™ spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

The IP26 antibody reacts with a monomorphic determinant of the α/β T-cell receptor, which is expressed on greater than 95% of normal peripheral blood CD3+ T cells. The α/β TCR recognizes a peptide bound to MHC leading to T-cell activation.

Other Names: α/β TCR, TCR α/β
Structure: Ig superfamily, with CD3 forms CD3/TCR complex
Distribution: T cells, thymocytes
Function: Antigen recognition, T cell activation
Ligand Receptor: Peptide bound to MHC
Antigen
References:

1. Marchalonis J, et al. 2002. J. Mol. Recognit. 15:260.

GeneID: 6955
6957
Latest Publications: View the latest TCR alpha/beta articles on HighwirePress.com
UniProt: View information about TCR alpha/beta on UniProt.org
Keywords: Brilliant Violet 421™ anti-human TCR α/β, IP26, Brilliant Violet 421™, BV421, α/β TCR, TCR α/β, Human, Flow Cytometry, Immunology, Antibodies


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Product FAQs

 
What is the F/P ratio range of our BV421 format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
For a complete list of FAQs on all our products, visit our FAQ page.
 
Related Products

DescriptionCloneApplications
Brilliant Violet 421™ Mouse IgG1, κ Isotype CtrlMOPC-21FC, ICFC, IF
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC
Human TruStain FcX™ (Fc Receptor Blocking Solution)FC, ICC, ICFC


Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-human TCR α/βIP26FC
Alexa Fluor® 647 anti-human TCR α/βIP26FC
APC anti-human TCR α/βIP26FC
Biotin anti-human TCR α/βIP26FC
FITC anti-human TCR α/βIP26FC
Pacific Blue™ anti-human TCR α/βIP26FC
PE anti-human α/β T Cell ReceptorIP26FC
PE/Cy5 anti-human TCR α/βIP26FC
PE/Cy7 anti-human TCR α/βIP26FC
PerCP/Cy5.5 anti-human TCR α/βIP26FC
Purified anti-human TCR α/βIP26FC


Related Pathways

Pathway
Gamma-Delta T cells
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Human Immunology > Th17 > α/β T Cell Receptor > IP26
Immunology > Human Immunology > Stem Cells > α/β T Cell Receptor > IP26
Immunology > Human Immunology > T Cell Receptors (TCRs) > αβ T Cell Receptor > IP26
Immunology > Human Immunology > Th1/Th2 > α/β T Cell Receptor > IP26
Immunology > Human Immunology > Innate Immunity > T Cell Receptors
Immunology > Human Immunology > T Regulatory Cells (TReg) > T Cell Receptors

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2013-05-14
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated IP26, followed by Sav-PE

  • FITC anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 FITC

  • PE anti-human α/β T Cell Receptor
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 PE

  • PE/Cy5 anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 PE/Cy5

  • Purified anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified IP26, then detected with anti-mouse IgG FITC

  • PE/Cy7 anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD19 (HIB19) FITC and IP26 PE/Cy7 (top) or mouse IgG1, κ PE/Cy7 isotype control (bottom)





  • Alexa Fluor® 488 anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 Alexa Fluor® 647

  • Pacific Blue™ anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 Pacific Blue™

  • APC anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 APC

  • Brilliant Violet 421™ anti-human TCR α/β
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-human TCRα/β (clone IP26) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).

  • PerCP/Cy5.5 anti-human TCR α/β
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-human TCR α/β (clone IP26) PerCP/Cy5.5 (filled histogram) or mouse IgG1, κ PerCP/Cy5.5 isotype control (open histogram).

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