Antibodies
      
 
Product Search  
 
Brilliant Violet 421™ anti-human TCR α/β Antibody
Cat. # Size Price  

306721 25 tests $185.00
    
306722 100 tests $385.00
    
Product Details

Clone: IP26 (See other available formats)
Isotype: Mouse IgG1, κ
Isotype Control:Brilliant Violet 421™ Mouse IgG1, κ Isotype Ctrl
Reactivity: Human
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
  Learn more about Brilliant Violet™

For a guide on how to use Brilliant Violet™ conjugates in flow cytometry, download our technical sheet: Brilliant Violet™ Considerations for Multicolor Flow Cytometry.
Excitation
Laser:
Violet Laser (405 nm)
Application
Notes:
Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone.  Other anti-CD3 clones may compete out the binding of IP26.  
Application
References:
  Publication Library
 Image 1
Human peripheral blood lymphocytes were stained with anti-human TCRα/β (clone IP26) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).


Compare all formats

See Brilliant Violet 421™ spectral data





 


Antigen Details

Description:

The IP26 antibody reacts with a monomorphic determinant of the α/β T-cell receptor, which is expressed on greater than 95% of normal peripheral blood CD3+ T cells. The α/β TCR recognizes a peptide bound to MHC leading to T-cell activation.

Other Names: α/β TCR, TCR α/β
Structure: Ig superfamily, with CD3 forms CD3/TCR complex
Distribution: T cells, thymocytes
Function: Antigen recognition, T cell activation
Ligand Receptor: Peptide bound to MHC
Antigen
References:
1. Marchalonis J, et al. 2002. J. Mol. Recognit. 15:260.
GeneID: 6955
6957
UniProt: View information about TCR alpha/beta on UniProt.org
Keywords: Brilliant Violet 421™ anti-human TCR α/β, IP26, Brilliant Violet 421™, BV421, α/β TCR, TCR α/β, Human, Flow Cytometry, Immunology, Antibodies
Technical Data Sheet (pdf)
Material Safety Data Sheet
Certificate of Analysis
Request Bulk Quote
Request Custom Conjugation
Email Technical Support
Write a Review
View All TCR α/β Reagents
Related Protocols

Cell Surface Immunofluorescence Staining Protocol
Rating

This product has not been reviewed yet.
Related Pages

Brilliant Violet™
Brilliant Violet™ Mobile Application
Multicolor Panel Selector
Fluorescence Spectra Analyzer
Fluorophore Brightness Index
Custom Panel Construction
Fluorophore Equivalency Tool
Flow Cytometry Mobile Application
Fluorescence Spectra Analyzer Mobile Application

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2013-05-14
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with biotinylated IP26, followed by Sav-PE

  • FITC anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 FITC

  • PE anti-human α/β T Cell Receptor
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 PE

  • PE/Cy5 anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 PE/Cy5

  • Purified anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with purified IP26, then detected with anti-mouse IgG FITC

  • PE/Cy7 anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with CD19 (HIB19) FITC and IP26 PE/Cy7 (top) or mouse IgG1, κ PE/Cy7 isotype control (bottom)





  • Alexa Fluor® 488 anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 Alexa Fluor® 488

  • Alexa Fluor® 647 anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 Alexa Fluor® 647

  • Pacific Blue™ anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 Pacific Blue™

  • APC anti-human TCR α/β
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with IP26 APC

  • Brilliant Violet 421™ anti-human TCR α/β
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-human TCRα/β (clone IP26) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).

  • PerCP/Cy5.5 anti-human TCR α/β
    Human peripheral blood lymphocytes were

    Human peripheral blood lymphocytes were stained with anti-human TCR α/β (clone IP26) PerCP/Cy5.5 (filled histogram) or mouse IgG1, κ PerCP/Cy5.5 isotype control (open histogram).

x
x
*Your Rating:
*Your Comment:
*Name (first, last):
*Affiliation:
*Email:
*Required fields
Note: Submission of a quick review does not qualify for our promotional $25 Amazon gift card. A full review submission is required for gift card eligibility.

Learn more…
9727 Pacific Heights Blvd.
San Diego, CA 92121

Tel: 858.768.5800
Company
Careers
Promotions
Web Tools
Areas of Biology
Pathways
Products
Literature
Custom Products/Services
Custom Panel Construction
Technical Support
Support Resources
Ordering
Contact
Distributors
Sitemap
Connect with us:

Facebook Twitter Pinterest LinkedIn YouTube Tumblr Blog Podcast
The LEGENDeers Pandora Google+ Wikipedia RSS Feed Arcade Words With Elements Satisfaction Survey
Privacy Policy | Terms
World-Class Quality | Superior Customer Support | Outstanding Value Copyright © 2014 BioLegend, Inc. All rights reserved.
Product Search