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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections3 and costimulation of T cell responses4. The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 301018).
Application References:
1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 3. Mack CL, et al. 2004. Pediatr. Res. 56:79. 4. Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022. 5. Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed 5. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed 6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed 7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) 8. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
Human peripheral blood lymphocytes were stained with CD3 FITC and CD8 (clone RPA-T8) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.
Other Names:
T8, Leu2
Structure:
Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
Distribution:
Majority of thymocytes, T cell subset, NK cells
Function:
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand Receptor:
MHC Class I molecules
Antigen References:
1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 APC
APC/Cy7 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 APC/Cy7
Biotin anti-human CD8a
Human peripheral blood lymphocytes stained with biotinylated RPA-T8, followed by Sav-PE
FITC anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 FITC
LEAF™ Purified anti-human CD8a
Human peripheral blood lymphocytes stained with LEAF™ purified RPA-T8, followed by anti-mouse IgGs FITC
PE anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 PE
PE/Cy5 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 PE
PE/Cy7 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 PE/Cy7
Purified anti-human CD8a
Human peripheral blood lymphocytes stained with purified RPA-T8, followed by anti-mouse IgGs FITC
Alexa Fluor® 647 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 Alexa Fluor® 647
Pacific Blue™ anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 Pacific Blue™
Alexa Fluor® 700 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 Alexa Fluor® 700
PerCP anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 PerCP
PerCP/Cy5.5 anti-human CD8a
Human peripheral blood lymphocytes stained with RPA-T8 PerCP/Cy5.5
Brilliant Violet 421™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FITC and CD8 (clone RPA-T8) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 570™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 and CD8 (clone RPA-T8) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).
Human RBC-lysed whole blood cells were stained with anti-CD8 (clone RPA-T8) BV570™ and anti-CD56 (clone HCD56) BV421™. Cells were gated on the lymphocyte population.
Brilliant Violet 605™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FITC and CD8 (clone RPA-T8) Brilliant Violet 605™.
Brilliant Violet 650™ anti-human CD8a
Human peripheral blood lymphocytes were stained with CD3 FITC and CD8 (clone RPA-T8) Brilliant Violet 650™.
