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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Human peripheral blood lymphocytes were stained with CD3 FITC and CD73 (clone AD2) Brilliant Violet 421™ or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T- and B-cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.
Subsets of T- and B-cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells
Function:
Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.
Antigen References:
1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication. 2. Airas L and Jalkanen S, et al. 1996. Blood 88:1755. 3. Gutensohn W, et al. 1995. Cell Immunol. 161:213. 4. Airas L, et al. 1995. J. Exp. Med. 182:1603.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Human peripheral blood lymphocytes were stained with CD3 FITC and CD73 (clone AD2) Brilliant Violet 421™ or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
Purified anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes stained with AD2 PE and CD3 APC
Human peripheral blood lymphocytes stained with AD2 PE and CD19 APC
PE anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes stained with AD2 PE and CD3 APC
Human peripheral blood lymphocytes stained with AD2 PE and CD19 APC
APC anti-human CD73 (Ecto-5'-nucleotidase)
Human peripheral blood lymphocytes stained with AD2 APC and CD19 PE (top) or mouse IgG1, k APC isotype control and CD19 PE (bottom).
