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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen tissue sections, and immunofluorescence microscopy3.
Application References:
1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC) 3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF) 4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496. 5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed 6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed 7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Human peripheral blood lymphocytes were stimulated with PMA + ionomycin for 6 hours and then stained with CD69 (clone FN50) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.
Other Names:
Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Structure:
C-type lectin, type II glycoprotein, 28/32 kD
Distribution:
Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells
Function:
Lymphocyte, monocyte, and platelet activation, NK cell killing
Antigen References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Testi R, et al. 1994. Immunol. Today 15:479.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD69
PHA-activated human peripheral blood lymphocytes stained with purified FN50, followed by anti-mouse IgGs FITC
FITC anti-human CD69
PMA + ionomycin stimulated (6 hours) human lymphocytes stained with FN50 FITC
PE anti-human CD69
PMA + ionomycin stimulated (6
hours) human lymphocytes stained
with FN50 PE
PE/Cy5 anti-human CD69
PHA-activated human peripheral blood lymphocytes stained with FN50 PE/Cy5
APC anti-human CD69
PMA+ionomycin-stimulated (5hours) human peripheral blood lymphocytes stained with FN50 APC
APC/Cy7 anti-human CD69
PMA + ionomycin stimulated (6 hours) human lymphocytes stained with FN50 APC/Cy7
PE/Cy7 anti-human CD69
PMA+ionomycin activated human peripheral blood lymphocytes stained with FN50 PE/Cy7
Alexa Fluor® 488 anti-human CD69
PMA+ionomycin activated human peripheral blood lymphocytes stained with FN50 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD69
PMA+ionomycin activated human peripheral blood lymphocytes stained with FN50 Alexa Fluor® 647
Pacific Blue™ anti-human CD69
PMA+ionomycin-stimulated human peripheral blood mononuclear cells (6 hours) stained with FN50 Pacific Blue™
Alexa Fluor® 700 anti-human CD69
PMA + Ionomycin-stimulated (5 hours) human peripheral blood lymphocytes stained with FN50 Alexa Fluor® 700
Biotin anti-human CD69
PHA-stimulated human peripheral blood mononuclear cells (day-2) stained with biotinylated FN50, followed by Sav-PE
PerCP/Cy5.5 anti-human CD69
PMA+ionomycin-stimulated (5 hours) human peripheral blood lymphocytes stained with FN50 PerCP/Cy5.5
PerCP anti-human CD69
PMA + Inonomycin-stimulated (5 hours) human peripheral blood lymphocytes were stained with anti-human CD69 (clone FN50) PerCP (filled histogram) or mouse IgG1, κ PerCP isotype control (open histogram).
Brilliant Violet 421™ anti-human CD69
Human peripheral blood lymphocytes were stimulated with PMA + ionomycin for 6 hours and then stained with CD69 (clone FN50) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
