Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Additional reported applications (for the relevant formats) include: mediating re-directed NK cell cytotoxicity.
Application References:
1. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309. 2. Kalberer CP, et al. 2003. Blood 102:127. 3. Chen Y, et al. 2007. J. Immunol. 179:2766. 4. Jarahain M, et al. 2009. J. Virol. 83:8108. PubMed
Human peripheral blood lymphocytes were stained with CD56 APC and CD335 (clone 9E2) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which trigger cytotoxicity in NK cells. CD335 is directly involved in target cell recognition and lysis and is exclusively expressed on CD3-CD56+ NK cells, suggesting it to be a universal marker for NK cells. NKp46 along with NKp30 and NKp44 are referred to as natural cytoxicity receptors (NCR) and play a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.
Other Names:
NKp46, NCR1
Structure:
Type I membrane glycoprotein (46 kD)
Distribution:
Expressed on resting and activated NK cells
Antigen References:
1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147. 2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309. 3. Sivori S. 1999. Eur. J. Immunol. 29:1656.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD335 (NKp46)
Human peripheral blood lymphocytes stained with purified 9E2, followed by anti-mouse IgG FITC
Biotin anti-human CD335 (NKp46)
Human peripheral blood lymphocytes stained with 9E2 biotin, followed by Sav-PE
PE anti-human CD335 (NKp46)
Human peripheral blood lymphocytes stained with CD56 (HCD56) APC and 9E2 PE
Alexa Fluor® 647 anti-human CD335 (NKp46)
Human peripheral blood lymphocytes stained with 9E2 Alexa fluor® 647
Pacific Blue™ anti-human CD335 (NKp46)
Human peripheral blood lymphocytes stained with 9E2 Pacific Blue™ and CD56 (HCD56) PE
Brilliant Violet 421™ anti-human CD335 (NKp46)
Human peripheral blood lymphocytes were stained with CD56 APC and CD335 (clone 9E2) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
