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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Additional reported applications (for the relevant formats) include: immunoprecipitation, Westrn blotting3, and induction of cytokine production3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 303410).
Application References:
1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. 2. Favaloro E, et al. 1988. Br. J. Haematol. 69:163. 3. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
Human peripheral blood monocytes were stained with CD33 (clone WM53) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.
Other Names:
Siglec-3, gp67, p67
Structure:
Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD33
Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with CD33 (clone WM53) APC.
PE anti-human CD33
Human peripheral blood lymphocytes,
monocytes and granulocytes
stained with WM53 PE
PE/Cy5 anti-human CD33
Human peripheral blood lymphocytes,
monocytes and granulocytes
stained with WM53 PE
Purified anti-human CD33
Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with purified CD33 (clone WM53), followed by anti-mouse IgGs FITC.
LEAF™ Purified anti-human CD33
Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with LEAF™ purified CD33 (clone WM53), followed by anti-mouse IgGs FITC.
PerCP/Cy5.5 anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clone WM53) PerCP/Cy5.5 (red histogram), or mouse IgG1, κ PerCP/Cy5.5 (green histogram).
Brilliant Violet 421™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clone WM53) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
Brilliant Violet 570™ anti-human CD33
Human peripheral blood monocytes were stained with CD33 (clone WM53) Brilliant Violet 570™ (filled histogram) or mouse IgG1, κ Brilliant Violet 570™ isotype control (open histogram).
