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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Additional reported applications (for the revelant formats) include: immunofluorescence and immunohistochemistry3.
Application References:
1. Lammers R, et al. 2002. Exp. Hematol. 30:537. 2. Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed 3. Human Protein Atlas http://hpr-external.genpat.uu.se (IHC)
Human colon carcinoma cell line HT29 was stained with 9C4 Brilliant Violet 421™ (filled histogram) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
CD326 is known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40kD protein is highly expressed in the bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. The CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.
Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
Distribution:
Highly expressed in the bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.
Function:
Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Modification:
Glycosylated
Antigen References:
1. Strnad J, et al. 1989. Cancer Res. 49:314. 2. Munz M, et al. 2004. Oncogene 23:5748. 3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD326 (EpCAM)
Human colon carcinoma cell line HT29 stained with purified 9C4, followed by anti-mouse IgG FITC
FITC anti-human CD326 (EpCAM)
Human colon carcinoma cell line HT29 stained with 9C4 FITC
PE anti-human CD326 (EpCAM)
Human colon carcinoma cell line (HT29) stainined with 9C4 PE
APC anti-human CD326 (EpCAM)
Human colon carcinoma cell line HT29 stained with 9C4 APC
Alexa Fluor® 488 anti-human CD326 (EpCAM)
Human colon carcinoma cell line HT29 stained with 9C4 Alexa Fluor® 488
Alexa Fluor® 647 anti-human CD326 (EpCAM)
Human colon carcinoma cell line (HT29) stainined with 9C4 Alexa Fluor® 647
PerCP/Cy5.5 anti-human CD326 (EpCAM)
Human colon carcinoma cell line HT29 stained with 9C4 PerCP/Cy5.5
Biotin anti-human CD326 (EpCAM)
Human colon carcinoma cell line (HT29) stained with biotinylated 9C4, followed by Sav-PE
Pacific Blue™ anti-human CD326 (EpCAM)
Human colon carcinoma cell line (HT29) stained with 9C4 Pacific Blue™
Brilliant Violet 421™ anti-human CD326 (EpCAM)
Human colon carcinoma cell line HT29 was stained with 9C4 Brilliant Violet 421™ (filled histogram) or mouse IgG2b, κ Brilliant Violet 421™ isotype control (open histogram).
