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The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Additional reported applications (for the relevant formats) include: inhibition of cytokine production and Western blotting under nonreducing condition.
Application References:
1. Gumá M, et al. 2004. Blood 104:3664. 2. Exley M, et al. 1998. J. Exp. Med. 188:867. 3. Marquez C, et al. 1998. Blood 91:2760.
Human peripheral blood lymphocytes stained with CD4 FITC and CD161 (clone HP-3G10) Brilliant Violet 421™ (top) or mouse IgG1 κ Brilliant Violet 421™ isotype control (bottom).
CD161 is a type II transmembrane glycoprotein, also known as NKR-P1A, that is expressed as a 40-44 kD homodimer. It is a member of the C-type lectin superfamily. CD161 is expressed on a majority of NK cells, NKT cells, and subsets of peripheral T cells and CD3+ thymocytes. It has been reported that Th17 cells are a subpopulation of CD4+CD161+CCR6+ cells. While the biological function of CD161 is not clear, it has been suggested to serve either as a stimulatory receptor or to inhibit NK cell-mediated cytotoxicity and cytokine production. LLT-1 (lectin-like transcript-1, also named as osteoclast inhibitory lectin or CLEC2D) is the ligand of CD161.
Other Names:
NKR-P1A
Structure:
Type II glycoprotein, 40-44 kD homodimer, C-type lectin superfamily
Distribution:
NK cells, T subset, subset of CD3+ thymocytes
Ligand Receptor:
LLT-1 (Lectin-like transcript-1)
Antigen References:
1. Takahashi T, et al. 2006. J. Immunol. 176:211. 2. Cosmi L, et al. 2008. J. Exp. Med. 205:1903. 3. Aldemir H, et al. 2005. J. Immunol. 175:7791. 4. Rosen DB, et al. 2008. J. Immunol. 180:6508.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD161
Human peripheral blood lymphocytes stained with HP-3G10 FITC and anti-CD4 (RPA-T4) PE
PE anti-human CD161
Human peripheral blood lymphocytes stained with CD4 FITC and HP-3G10 PE
Human peripheral blood CD4+ lymphocytes stained with CCR6 PerCP/Cy5.5 and HP-3G10 PE
PerCP/Cy5.5 anti-human CD161
Human peripheral blood lymphocytes stained with HP-3G10 PerCP/Cy5.5 and anti-CD4 (RPA-T4) PE
Alexa Fluor® 647 anti-human CD161
Human peripheral blood lymphocytes stained with HP-3G10 Alexa Fluor® 647 and CD4 (RPA-T4) PE
APC anti-human CD161
Human peripheral blood lymphocytes were stained with CD4 FITC, CCR6 PE and CD161 (clone HP-3G10) APC (top) or mouse IgG1, κ APC isotype control (bottom). Data shown was gated on the CD4+ population.
Brilliant Violet 421™ anti-human CD161
Human peripheral blood lymphocytes stained with CD4 FITC and CD161 (clone HP-3G10) Brilliant Violet 421™ (top) or mouse IgG1 κ Brilliant Violet 421™ isotype control (bottom).
Brilliant Violet 605™ anti-human CD161
Human peripheral blood lymphocytes were stained with CD4 FITC and CD161 (clone HP-3G10) Brilliant Violet 605™.
PE/Cy7 anti-human CD161
Human peripheral blood lymphocytes were stained with CD4 FITC and CD161 (clone HP-3G10) PE/Cy7 (top) or mouse IgG1, κ PE/Cy7 isotype control (bottom).
