BioLegend Brightness Index
Fluorophore Ex (nm) Max Em (nm) Max Filter Used Brightness Histogram
PE 565 575 585/20 5
PE/Cy5 565 670 660/20 5
APC 650 660 660/20 5
PE/Dazzle™ 594 565 610 610/20 5
Brilliant Violet 421™ 405 421 450/50 5
PE/Cy7 565 774 780/60 4
Brilliant Violet 605™ 405 603 610/20 4
Brilliant Violet 711™ 405 711 710/50 4
Alexa Fluor® 647 650 668 660/20 4
Brilliant Violet 650™ 405 645 660/20 4
Brilliant Violet 785™ 405 785 780/60 3
Brilliant Violet 510™ 405 510 510/50 3
Brilliant Violet 570™ 405 570 585/42 3
Alexa Fluor® 488 495 519 530/30 3
PerCP/Cy5.5 482 690 695/40 2
APC/Cy7 650 774 780/60 2
Pacific Blue™ 410 455 450/50 2
FITC 493 525 530/30 2
APC/Fire™ 750 650 787 780/60 2
Alexa Fluor® 700 696 719 720/45 1
PerCP 482 675 695/40 1
Data images show anti-mouse CD19 (clone 6D5) staining of mouse splenocytes with the appropriate fluorophore direct conjugate. Flow cytometry was performed on a BD LSRFortessa™.
The Fluorophore Brightness Index Score is a relative indication of fluorescence intensity above the background for each fluorophore antibody conjugation (1=dim, 5=brightest). These values can differ depending upon the flow cytometer, the instrument filters and settings, laser power, the antibody clone and the antigen target, f/p ratios, the buffer conditions, etc.
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