BioLegend Biomarkers and Immunoassays
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BioLegend's LEGENDplex™ bead-based immunoassays quantify multiple soluble analytes simultaneously in biological samples using a flow cytometer. The assay utilizes the same basic principles of sandwich immunoassays, whereby a soluble analyte is captured between two antibodies.

LEGENDplex™ kits are provided with predefined panels, ranging from 3 to 13 specificities, or customers can mix and match any subset within each predefined panel using our Mix and Match system. Custom options are also available.

Each kit provides sufficient reagents for at least 100 tests. The assay can be performed in a 96-well filter plate or v-bottom plate, allowing you to run 40 samples plus 8 standard curve titrations in duplicate, or they can be performed in FACS tubes, where duplicate titrations may not be necessary.

Software is downloaded for free and each kit comes with a dongle key required for use.

Learn more about LEGENDplex™
LEGENDplex™ Predefined Panels

The LEGENDplex™ panels cover a wide range of soluble markers for immunology, growth factors, and metabolism. Currently, reactivities include human, mouse, rat, and non-human primate species (Rhesus and Cynomolgus). Panels are carefully selected for important biomarkers that are often analyzed together in a set.
T Helper Cytokine Panels
Proinflammatory Chemokine Panel
Cytokine Panel 2
Inflammation Panel
Growth Factor Panel
Adipokine Panel
Metabolic Panel 1
CD8/NK Panel
Anti-Virus Response Panel
Apolipoprotein (Apo) Panel
Free Active/Total TGF-β1 Panel (Human/NHP)
TIMP Panel
B Cell Panels
Macrophage/Microglia Panels
Kidney Function Panel 1, for Urine Samples
Kidney Function Panel 2, for Serum/Plasma Samples
Kidney Function Panel 3, for Urine/Serum/Plasma Samples
Vascular Inflammation Panel 1
Hematopoietic Stem Cell Panel
Immunoglobulin Isotyping Panel
Bone Metabolism Panel 1
Angiogenesis Panel 1
Human Fibrinolysis Panel
Inflammation Panel 2
Thrombosis Panel
Neuroinflammation Panel 1
Inflammation Panel 1
Adhesion Molecule Panel
Diabesity Panel
T Helper Cytokine Panels
Proinflammatory Chemokine Panel
Cytokine Panel 2
Inflammation Panel
Immunoglobulin Isotyping Panel
Free Active/Total TGF-β1 Panel (Mouse/Rat)
Anti-Virus Response Panel
HSC Panel
IgE Panel
T Helper Cytokine Panel Version 2
Macrophage/Microglia Panel
T Helper Cytokine Panels
Chemokine/Cytokine Panel
Inflammation Panel
Growth Factor Panel
Adipokine Panel
T Helper Cytokine Panels
Inflammation Panel
View panel details…
LEGENDplex™ Mix and Match

Our Mix and Match system is more suited for researchers requiring flexibility in the in their choice of analytes. Instead of ordering all 13 specificities in a predefined panel, you can choose any subset within a panel, even a single analyte if desired. The system is easy to use and cost-effective. Just order the analyte capture beads, the appropriate lyophilized standard, the appropriate detection antibodies, and the appropriate buffer set.

Mix and Match kits must be ordered using the LEGENDplex™ page.

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