The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
This reagent has been tested by immunofluorescent staining and can be used at ≤ 0.5 ug/million cells. In addition, it has been tested by ELISA (≤ 2 ug/ml) to assure the specificity and reactivity. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
This biotinylated polyclonal goat anti-rat IgG antibody is useful for ELISA, Western blot, IHC, and immunofluorescent staining for flow cytometry.
Description:
This polyclonal goat anti-rat IgG antibody reacts with the heavy chains of rat IgG and with the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with mouse, human, bovine, horse, and rabbit immunoglobulins, but it may cross-react with other subclasses of rat immunoglobulins.
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