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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, blocking of costimulation of T cells1-3, and immunohistochemical staining of acetone-fixed frozen sections3. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200310).
CD86 is an 80 kD member of the immunoglobulin superfamily also known as B70 or B7-2. It is expressed on antigen presenting cells and activated T and B cells. CD86 is a ligand for CD28 and CD152 (CTLA-4). It is one of the accessory molecules that plays an important role in T cell-APC cell costimulatory interactions. The 24F antibody can block the co-stimulatory activity of CD86.
Other Names:
B7-2, B70
Structure:
Ig superfamily, 80 kD
Distribution:
Antigen presenting cells, activated B and T cells
Function:
T and B cell costimulation
Ligand Receptor:
CD28, CD152 (CTLA-4)
Antigen References:
1. Barclay, A.N. et al. 1997. The Leukocyte Antigen FactsBook. Academic Press. 2. Hathcock, K.S., et al. 1993. Science 262:905. 3. Freeman, G.J., et al. 1993. Science 262:907. 4. Carreno, B.M., et al. 2002. Ann. Rev. Immunol. 20:29.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE anti-rat CD86
LPS-stimulated (3 days) LOU rat splenocytes stained with 24F PE
FITC anti-rat CD86
ConA-stimulated (3 days) LOU rat splenocytes stained with 24F FITC
Biotin anti-rat CD86
LPS-stimulated (3 days) LOU rat splenocytes stained with biotinylated 24F, followed by SAV-PE
Alexa Fluor® 647 anti-rat CD86
ConA-stimulated (3 days) LOU rat splenocytes stained with 24F Alexa Fluor® 647
