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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of C3bi binding3,4.
Application References:
1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC) 2. Milligan CE, et al. 1991. J. Comp. Neurol. 314:125. (IHC) 3. Robinson AP, et al. 1986. Immunology 57:239. (Block) 4. Issekutz SE, et al. 1992. Immunology 76:655. (Block) 5. Muehlbauer SM, et al. Am. J Pathol. 177:735. (FC) PubMed
LLOU rat bone marrow cells stained with biotinylated OX-42, followed by Sav-PE (gated on myeloid cells)
LOU rat splenocytes stained with biotinylated OX-42, followed by Sav-PE
The OX-42 antibody reacts with the CR3 complement (C3bi) receptor expressed on monocytes, granulocytes, macrophages, dendritic cells, NK cells, and a subset of lymphocytes. This antibody appears to recognize a common epitope shared between CD11b and CD11c (integrin αM and αX chains). The OX-42 antibody precipitates three polypeptides with apparent molecular weights of 160, 103, and 95 kD, respectively. This antibody has been shown to block the formation of complement-mediated rosettes and leukocyte migration.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-rat CD11b/c
Lou splenocytes stained with purified OX-42, followed by anti-mouse IgG FITC
FITC anti-rat CD11b/c
LOU rat splenocytes stained with OX-42 FITC
PE anti-rat CD11b/c
LOU rat splenocytes stained with OX-42 PE
APC anti-rat CD11b/c
LOU rat bone marrow cells stained with OX-42 APC (gated on myeloid cell population)
Biotin anti-rat CD11b/c
LLOU rat bone marrow cells stained with biotinylated OX-42, followed by Sav-PE (gated on myeloid cells)
LOU rat splenocytes stained with biotinylated OX-42, followed by Sav-PE
Alexa Fluor® 647 anti-rat CD11b/c
LOU rat splenocytes stained with OX-42 Alexa Fluor® 647
