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PHA-stimulated rat splenocytes and rat thymic lymphoma FTL-43
Formulation:
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.5% BSA (Origin USA)
Preparation:
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The WT.1 antibody recognizes both activated and unactivated LFA-1 and inhibits the binding of LFA-1 to ICAM-1. The WT.1 antibody has been shown to inhibit the mixed lymphocyte reaction, leukocyte infiltration, and graft rejection. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of cellular adhesion3-6.
Application References:
1. Andrews FJ, et al. 1997. Dig. Dis. Sci. 42:326. (IHC) 2. Springer TA. 1994. Cell 76:301. (IHC) 3. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. (IP Block) 4. Devine L, et al. 1996. Immunol. 88:456. (Block) 5. Ohta Y, et al. 1998. Transplantation 65:1094. (Block) 6. Watanabe T, et al. 1997. Am. J. Pathol. 150:971. (Block)
LOU rat splenocytes stained with biotinylated WT.1, followed by Sav-PE
CD11a is a 160-170 kD glycoprotein also known as αL integrin, or LFA-1 α chain. It is a member of the integrin family and forms a heterodimer with β2 integrin (CD18). The LFA-1 (CD11a/CD18) is broadly expressed on leukocytes and mediates intercellular adhesion and cellular activation. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are the ligands of LFA-1.
Other Names:
LFA-1 α chain, αL Integrin
Structure:
Integrin family member, 160-170 kD, associates with integrin β2
Distribution:
Lymphocytes, granulocytes, monocytes, macrophages
Function:
Cellular adhesion
Ligand Receptor:
ICAM-1, ICAM-2, ICAM-3
Antigen References:
1. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. 2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:855.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-rat CD11a
LOU rat splenocytes stained with purified WT.1, followed by anti-mouse IgG2a FITC
Biotin anti-rat CD11a
LOU rat splenocytes stained with biotinylated WT.1, followed by Sav-PE
