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The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin and unconjugated antibody.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen skin and intestine epithelium1,4, immunocytochemical staining of cytospin preparations4, and Western blot1.
Application References:
1. Brenan M, et al. 1992. J. Exp. Med. 175:1457. (IHC, WB) 2. Chen-Woan M, et al. 1995. J. Immunol. Methods 178:157. 3. Chen-Woan M, et al. 1996. J. Leuk. Biol. 59:196. 4. Brenan M and Rees DJ. 2000. Eur. J. Immunol. 30:1527. (IHC)
LOU rat intestinal intraepithelial lymphocytes were stained with CD3 Alexa Fluor® 647 and biotinylated CD103 (clone OX-62, top) or mouse IgG1, κ isotype control (bottom), followed by SAV-PE.
CD103, also known as Integrin αE or integrin αIEL, is a single pass transmembrane protein composed by two chains, heavy and light, linked by a disulfide bond. It is expressed in the cell surface as an αE/β7 heterodimer. CD103 mediates adhesion of intraepithelial T cells (IEL) to the epithelium. CD103 is expressed by IEL, effector and memory Tregs, and dendritic cells and their immediate precursors. E-cadherin is the ligand for CD103.
Other Names:
Integrin αE, integrin E, integrin αIEL, integrin αOX-62, Itgae
Structure:
Single pass transmembrane protein, composed by two chains, heavy and light, linked by a disulfide bond. Associates with integrin β7 to form the αE/β7 complex.
Distribution:
Intraepithelial T lymphocytes, effector and memory Tregs, dendritic cells and their immediate precursors.
Function:
Mediates adhesion of intraepithelial T cells (IEL) to epithelium.
Ligand Receptor:
E-cadherin
Antigen References:
1. Varas A, et al. 1998. Blood 92:93. 2. Brissette-Storkus CS, et al. 2002. J. Leukoc. Biol. 71:941. 3. Hubert FX, et al. 2004. J. Immunol. 172:7485. 4. Tsuchihashi S, et al. 2006. J. Immunol. 176:616. 5. Lührmann A, et al. 2007. Immunol. Lett. 108:167.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Alexa Fluor® 488 anti-rat CD103 (αE Integrin)
LOU rat intestinal intraepithelial lymphocytes were stained with CD103 (clone OX-62) Alexa Fluor® 488 (top) or mouse IgG1, κ Alexa Fluor® 488 isotype control (bottom) and CD3 Alexa Fluor® 647.
FITC anti-rat CD103 (αE Integrin)
LOU rat intestinal intraepithelial lymphocytes were stained with CD3 Alexa Fluor® 647 and CD103 (clone OX-62) FITC (top) or mouse IgG1, κ FITC isotype control (bottom).
Alexa Fluor® 647 anti-rat CD103 (αE Integrin)
LOU rat intestinal intraepithelial lymphocytes were stained with CD3 FITC and CD103 (clone OX-62) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
Biotin anti-rat CD103 (αE Integrin)
LOU rat intestinal intraepithelial lymphocytes were stained with CD3 Alexa Fluor® 647 and biotinylated CD103 (clone OX-62, top) or mouse IgG1, κ isotype control (bottom), followed by SAV-PE.
Purified anti-rat CD103 (αE Integrin)
LOU rat intestinal intraepithelial lymphocytes were stained with CD3 Alexa Fluor® 647 and CD103 (clone OX-62) FITC (top) or mouse IgG1, κ FITC isotype control (bottom).
