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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Western blotting, suggested working dilution(s): Use 5 µg/5ml antibody dilution buffer per mini-gel. Do not use dilution or blocking buffers containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunofluorescence microscopy3.
Application References:
1. Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB) 2. Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB) 3. Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (IF) 4. Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phosphotyrosine antibody (clone PY-20). Lane 1, serum-starved Hela cells; Lane 2, serum-starved Hela cells following serum addition for 4 hrs. Lane 2 shows an upregulation of tyrosine phosphorylated proteins after serum addition. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.
Distribution:
Phospho-Specific
Function:
Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-Phosphotyrosine
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phosphotyrosine antibody (clone PY-20). Lane 1, serum-starved Hela cells; Lane 2, serum-starved Hela cells following serum addition for 4 hrs. Lane 2 shows an upregulation of tyrosine phosphorylated proteins after serum addition. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Purified anti-Phosphotyrosine
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-phosphotyrosine antibody (clone PY-20). Lane 1, serum-starved Hela cells; Lane 2, serum-starved Hela cells following serum addition for 4 hrs. Lane 2 shows an upregulation of tyrosine phosphorylated proteins after serum addition. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
