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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. Please follow the Cell Fixation and Permeabilization Protocol Using 70% Ethanol. For immunofluorescent staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, intracellular flow cytometry3, immunofluorescence microscopy9, and Western blotting10.
Application References:
1. Ogata K, et al. 1985. J. Immunol. 135:2623. 2. Garcia R, et al. 1989. Am. J. Pathol. 134:733. 3. Landberg G, et al. 1990. Exp. Cell. Res. 187:111. 4. Waseem N, et al. 1990. J. Cell Sci. 96:121. 5. Yu C, et al. 1991. Histopathology 19:29. 6. Wilkins B, et al. 1992. J. Pathol. 166:45. 7. Yang W, et al. 1996. Human Pathol. 27:70. 8. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329. 9. Chou HYE, et al. 2006. J. Biol. Chem. 10:1074. 10. Fulvio MD, et al. 2006. Oncogene 25:3932. 11. Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937.
Description:
The PC10 monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase δ auxiliary protein. PCNA is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase δ processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody.
Other Names:
Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein
RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
Ligand Receptor:
Ubiquitination, Sumoylation
Interaction:
PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
Antigen References:
1. Travali S, et al. 1989. J. Biol. Chem. 264:7466. 2. Waseem N, et al. 1990. J. Cell Sci. 96:121. 3. Hall P, et al. 1990. J. Pathol. 162:285. 4. Landberg G, et al. 1991. Cancer Res. 51:4570. 5. Woods A, et al. 1991. Histopathol. 19:21. 6. Hoege C, et al. 2002. Nature 419:135. 7. Yue H, et al. 2003. World J. Gastroenterol. 9:377. 8. Shan B, et al. 2003. J. Biol. Chem. 278:44009.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
