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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: blocking of ligand binding1-4, activation of αv β3 integrin signaling5, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 104310).
Application References:
1. Kieffer N, et al. 1990. Annu. Rev. Cell Biol. 6:329. (Block) 2. Piali L, et al. 1995. J. Cell Biol. 130:451. (Block) 3. Ashkar S, et al. 2000. Science 287:860. (Block) 4. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block) 5. Moulder K, et al. 1991. J. Exp. Med. 173:343. (Activ) 6. Carlson TR, et al. 2008.135:2193. PubMed 7. Yamaji D, et al. 2009. Genes Dev. 23:2382. PubMed
C57BL/6 mouse bone marrow cells stained with biotinylated 2C9.G2, followed by Sav-PE
CD61 is a 110 kD integrin β chain also known as β3 integrin or gpIIIa. It associates with the integrin αv chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin αIIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of αv/β3 integrin or vitronectin receptor to CD31 (PECAM-1). The 2C9.G2 antibody reacts with mouse and rat CD61, and is able to block ligand binding.
Other Names:
β3 integrin, Integrin β3 chain, gpIIIa
Structure:
Integrin family, 110 kD
Distribution:
Platelets, megakaryocytes, endothelium, smooth muscle, activated T cells, subset of B cells, monocytes, macrophages, osteoclasts, mast cells
Function:
Attachment to matrix
Ligand Receptor:
Fibrinogen, von Willebrand factor, fibronectin
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Phillips DR, et al. 1991. Cell 65:359. 3. Felding-Habermann B, et al. 1993. Curr. Opinion Cell Biol. 5:864.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with biotinylated 2C9.G2, followed by Sav-PE
FITC anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with HMβ3-1 FITC
LEAF™ Purified anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with LEAF™ purified 2C9.G2, followed by anti-Armenian hamster IgG FITC
PE anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with 2C9.G2 PE
Purified anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with purified 2C9.G2, followed by anti-Armenian hamster IgG FITC
Alexa Fluor® 488 anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with 2C9.G2 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse/rat CD61
C57BL/6 mouse bone marrow cells stained with 2C9.G2 Alexa Fluor® 647
