The IL-9 antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Storage & Handling:
The IL-9 antibody solution should be stored undiluted at 4°C. Do not freeze.
Application:
ELISA Detection1,2
Recommended Usage:
Each lot of this IL-9 antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 μg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-9 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
ELISA Detection1,2: The biotinylated D9302C12 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified D8402E8 antibody (Cat. No. 504702) as the capture antibody. Neutralization2,3: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for neutralization of mouse IL-9 bioactivity (Cat. No. 504802). Additional reported applications (for the relevant formats) include: Western blotting.
Application References:
1. Abrams, J. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. 2. Renauld, J., et al., 1995. J. Leuk. Biol. 57: 353. 3. Sitkauskiene, B., et al., 2005. Respir Res. 6: 33.
Description:
IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones. The D9302C12 antibody reacts with mouse IL-9. The D9302C12 antibody can neutralize the bioactivity of natural or recombinant mouse IL-9.
Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Cellular Sources:
IL-2 activated TH2 lymphocytes
Cellular Targets:
T lymphocytes, erythroid precursors
Receptors:
IL-9R
Bioactivity/Activities:
Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Antigen References:
1. Fitzgerald, K., et al., Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Quesniaux, V. 1992. Research Immunology 143:385. 3. Renauld, J., et al., 1993. Adv. Immunol. 54:79. 4. Yang, Y. 1992. Leuk. Lymphoma 8:441.
