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Biotin anti-mouse IgM Antibody
Biotin anti-mouse IgM Antibody
406503 50 µg $70.00     
406504 500 µg $170.00     
Product Details
Clone: RMM-1
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: mouse IgGs and IgM, IgE, IgA cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C. Do not freeze.
Application:

FC - Quality tested
ELISA - Reported in the literature
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
Notes:

Each lot of this antibody is quality control tested by immunofluorescent staining and flow cytometric analysis.
Application
References:

1. Tertilt C, et al. 2009. Infect Immun. 77:3044. (ELISA) PubMed
C57BL/6 Mouse splenocytes stained with
C57BL/6 Mouse splenocytes stained with biotinylated RMM-1, followed by Sav-PE


Compare all formats



Description:

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells. The RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Other Names: Immunoglobulin M
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
GeneID: 16019
Latest Publications: View the latest IgM articles on HighwirePress.com
UniProt: View information about IgM on UniProt.org
Keywords: Biotin anti-mouse IgM, RMM-1, Biotin, Immunoglobulin M, Mouse, Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-mouse IgM
    C57BL/6 Mouse splenocytes stained with

    C57BL/6 Mouse splenocytes stained with purified RMM-1, followed by anti-rat IgG FITC

  • PE anti-mouse IgM
    C57BL/6 splenocytes stained with RMM-1

    C57BL/6 splenocytes stained with RMM-1 PE and 6D5 (CD19) APC

  • Biotin anti-mouse IgM
    C57BL/6 Mouse splenocytes stained with

    C57BL/6 Mouse splenocytes stained with biotinylated RMM-1, followed by Sav-PE

  • FITC anti-mouse IgM
    C57BL/6 splenocytes stained with RMM-1

    C57BL/6 splenocytes stained with RMM-1 FITC

  • APC anti-mouse IgM
    C57BL/6 splenocytes stained with RMM-1

    C57BL/6 splenocytes stained with RMM-1 APC

  • PerCP/Cy5.5 anti-mouse IgM
    C57BL/6 splenocytes stained with RMM-1

    C57BL/6 splenocytes stained with RMM-1 PerCP/Cy5.5

  • APC/Cy7 anti-mouse IgM
    C57BL/6 splenocytes stained with CD19

    C57BL/6 splenocytes stained with CD19 (6D5) FITC and RMM-1 APC/Cy7

    C57BL/6 splenocytes stained with CD19

    C57BL/6 splenocytes stained with CD19 (6D5) FITC and rat IgG2a APC/Cy7 isotype control

  • Brilliant Violet 421™ anti-mouse IgM
    C57BL/6 splenocytes were stained with

    C57BL/6 splenocytes were stained with CD19 FITC and anti-IgM (clone RMM-1) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ isotype control (bottom).





  • PE/Cy7 anti-mouse IgM
    C57BL/6 splenocytes stained with CD19

    C57BL/6 splenocytes stained with CD19 (6D5) APC and RMM-1 PE/Cy7

    C57BL/6 splenocytes stained with CD19

    C57BL/6 splenocytes stained with CD19 (6D5) APC and rat IgG2a PE/Cy7 isotype control

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