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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The H-2Kd antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this H-2Kd antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, Western blotting2, and immunohistochemical staining of acetone-fixed frozen sections.
The SF1-1.1 antibody reacts with the H-2Kd MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kd haplotype. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The SF1-1.1 antibody is weakly cross-reactive with H-2k but does not cross-react with other haplotypes (b, j, p, q, s, v). Clone SF1-1.1 recognizes the α3 domain of Kd.
Other Names:
MHC class I
Structure:
MHC class I
Distribution:
Nucleated cells of the H-2Kd haplotype
Function:
Antigen presentation
Ligand Receptor:
CD3/TCR, CD8
Antigen References:
1. Watts C. 1997. Annu. Rev. Immunol. 15:821. 2. Pamer E, et al. 1998. Annu. Rev. Immunol. 16:323. 3. York I, et al. 1996. Annu. Rev. Immunol. 14:369.
Biotin anti-mouse H-2Kd, SF1-1.1, Biotin, MHC class I, Mouse d haplotype, Weakly cross-reacts with H-2k haplotype, Does not cross react with other haplotypes (e.g., b, j, p, q, s, v)., Flow Cytometry, Immunohistochemistry, Immunology, Antibodies
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse H-2Kd
Balb/c mouse splenocytes stained with biotinylated SF1-1.1, followed by Sav-PE
FITC anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 FITC
PE anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 PE
Purified anti-mouse H-2Kd
Balb/c mouse splenocytes stained with purified SF1-1.1, followed by anti-mouse IgG2a FITC
C57BL/6 mouse splenocytes stained with purified SF1-1.1, followed by anti-mouse IgG2a FITC
Alexa Fluor® 488 anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse H-2Kd
BALB/c mouse splenocytes stained with SF1-1.1 Alexa Fluor® 647
Pacific Blue™ anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 Pacific Blue™
C57BL/6 mouse splenocytes stained with SF1-1.1 Pacific Blue™
PerCP/Cy5.5 anti-mouse H-2Kd
Balb/c mouse splenocytes stained with SF1-1.1 PerCP/Cy5.5
