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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.
C57BL/6 mouse splenocytes were stained with biotinylated H-2Kb/H-2Db (clone 28-8-6) (filled histogram) or biotinylated mouse IgG2a, κ isotype control (open histogram), followed by Sav-PE.
The 28-8-6 antibody reacts with the H-2Kb and H-2Db MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Db haplotype. H-2Kb/H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2Dd MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.
Other Names:
MHC class I
Structure:
MHC class I
Distribution:
Nucleated cells of the H-2Kb/H-2Db haplotype
Function:
Antigen presentation
Ligand Receptor:
CD3/TCR, CD8
Antigen References:
1. Ozato K, et al. 1981. J. Immunol. 126:317. 2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447. 3. Evans GA, et al. 1992. Nature 300:755.
Biotin anti-mouse H-2K b/H-2D b, 28-8-6, Biotin, MHC class I, Mouse b haplotype, Cross-reacts with H-2Dd MHC class I alloantigen. Does not react with other haplotypes (e.g., f, k, p, q, r, s), Flow Cytometry, Immunology, Antibodies
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-mouse H-2Kb/H-2Db
C57BL/6 mouse splenocytes were stained with H-2Kb/H-2Db (clone 28-8-6) FITC (filled histogram) or mouse IgG2a, κ isotype control FITC (open histogram).
Purified anti-mouse H-2K b/H-2D b
C57BL/6 mouse splenocytes were stained with purified H-2Kb/H-2Db (clone 28-8-6) (filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a PE.
Biotin anti-mouse H-2K b/H-2D b
C57BL/6 mouse splenocytes were stained with biotinylated H-2Kb/H-2Db (clone 28-8-6) (filled histogram) or biotinylated mouse IgG2a, κ isotype control (open histogram), followed by Sav-PE.
PE anti-mouse H-2K b/H-2D b
C57BL/6 mouse splenocytes stained with 28-8-6 PE
Alexa Fluor® 647 anti-mouse H-2Kb/H-2Db
C57BL/6 mouse splenocytes stained with 28-8-6 Alexa Fluor® 647
