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Biotin anti-mouse DC Marker (33D1) Antibody
Biotin anti-mouse DC Marker (33D1) Antibody
124903 50 µg $60.00     
124904 500 µg $250.00     
Product Details
Clone: 33D1
Isotype: Rat IgG2b, κ
Isotype Control:Biotin Rat IgG2b, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: Mouse dendritic cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C. Do not freeze.
Application:

FC - Quality tested
IHC, IF - Reported in the literature
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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Application
References:

1. Crowley MT, et al. 1990. J Immunol Methods 133:55
2.Nussenzwig MC, et al. 1982. P. Natl. Acad. Sci. USA 79:161
BALB/c splenocytes stained with I-A/I-E
BALB/c splenocytes stained with I-A/I-E (M5/114) FITC and biotinylated 33D1, followed by Sav-PE


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Description:

33D1 antigen is reported to be a dendritic cell marker on subpopulation DC in spleen, thymus, Peyer’s patch. It is reported that most N418 (CD11c)+ cells in spleen are also 33D1 positive. Bone marrow dendritic cells upregulate 33D1 antigen expression after incubation with GM-CSF.
Other Names: Dendritic Cell Marker, 33D1
Structure: The antigen recognized by 33D1 is yet unknown.
Distribution: 33D1 antigen was reported on dendritic cell populations from mouse thymus, spleen lymph nodes and Peyer patches. Bone marrow dendritic cells can be induced to express 33D1 antigen in the presence of GM-CSF. In mouse spleen, most N418+ cells are also 33D1 positive.
Function: GM-CSF is reported to increase expression of 33D1 antigen on dendritic cells from bone marrow cells and IL-4 reported to down regulate the 33D1 antigen.
GeneID: 170779
Latest Publications: View the latest 33D1 articles on HighwirePress.com
UniProt: View information about 33D1 on UniProt.org
Keywords: Biotin anti-mouse DC Marker (33D1), 33D1, Biotin, Dendritic Cell Marker, 33D1, Mouse, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Biotin anti-mouse DC Marker (33D1)
    BALB/c splenocytes stained with I-A/I-E

    BALB/c splenocytes stained with I-A/I-E (M5/114) FITC and biotinylated 33D1, followed by Sav-PE

  • PE anti-mouse DC Marker (33D1)
    C57BL/6 splenocytes were stained with

    C57BL/6 splenocytes were stained with I-A/I-E APC and DC Marker (clone 33D1) PE (top) or rat IgG2b, κ PE isotype control (bottom).





  • FITC anti-mouse DC Marker (33D1)
    C57BL/6 splenocytes were stained with

    C57BL/6 splenocytes were stained with I-A/I-E APC and DC Marker (clone 33D1) FITC (top) or rat IgG2b, κ FITC isotype control (bottom).





  • Alexa Fluor® 488 anti-mouse DC Marker (33D1)
    C57BL/6 splenocytes stained with I-A/I-E

    C57BL/6 splenocytes stained with I-A/I-E (M5/114.15.2) APC and 33D1 Alexa Fluor® 488

    C57BL/6 splenocytes stained with I-A/I-E

    C57BL/6 splenocytes stained with I-A/I-E (M5/115) APC and Alexa Fluor® 488 rat IgG2b isotype control

  • Alexa Fluor® 647 anti-mouse DC Marker (33D1)
    C57BL/6 splenocytes stained with M5/114

    C57BL/6 splenocytes stained with M5/114 (I-A/I-E) PE and 33D1 Alexa Fluor® 647

  • APC anti-mouse DC Marker (33D1)
    C57BL/6 splenocytes were stained with

    C57BL/6 splenocytes were stained with I-A/I-E PE and DC Marker (clone 33D1) APC (top) or rat IgG2b, κ APC isotype control (bottom).





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