The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining2,6 of acetone-fixed frozen sections, immunofluorescence microscopy, and in vivo and in vitro blocking of T cell responses1-6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 105010).
Application References:
1. Hathcock, K.S., et al., 1993. Science 262:905. 2. Inaba, K., M., et al., 1994. J. Exp. Med. 180:1849. 3. Hathcock, K.S., et al., 1994. J. Exp. Med. 180:631. 4. Krummel, M.F., et al., 1995. J. Exp. Med. 182:459. 5. Liu, Y., et al., 1997. J. Exp. Med. 185:251. 6. Herold, K.C., et al., 1997. J. Immunol. 158:984. 7. Shih, F. F., et al., 2006. J. Immunol. 176:3438. 8. Lawson, B. R., et al., 2007. J. Immunol. 178:5366. 9. Turnquist, H.R., et al., 2007. J. Immunol. 178:7018.
C57BL/6 mouse splenocytes stained with biotinylated GL-1,followed by Sav-PE
Description:
CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The GL1 antibody can block the mixed lymphocyte reaction in vitro and has been shown to inhibit the priming of cytotoxic T lymphocytes in vivo (along with antibodies against B7-1).
Other Names:
B7-2, B70, Ly-58
Structure:
Ig superfamily, 80 kD
Distribution:
B and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
Function:
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand Receptor:
CD28, CD152 (CTLA-4)
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Hathcock, K.S., et al., 1993. Science 262:905. 3. Freeman, G.J., et al., 1993. Science 262:907. 4. Carreno, B.M., et al., 2002. Annu. Rev. Immunol. 20:29.
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