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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone - fixed frozen sections4.
CD83 is a 45 kD type I transmembrane protein. It belongs to immunoglobulin superfamily and is expressed on mature dendritic cells and activated lymphocytes. CD83 is involved in the regulation of T cell development and immune response. Soluble form CD83 has been reported to inhibit dendritic cell maturation and dendritic cell-mediated T cell proliferation. Murine CD83 ligand has been found on B cells.
Other Names:
HB15
Structure:
Type I transmembrane protein, Ig superfamily, 45kD
Distribution:
Mature dendritic cells, activated lymphocytes
Function:
Regulate T cell development and immune response, soluble form CD83 inhibit dendritic cell maturation
Ligand Receptor:
CD83 ligand
Antigen References:
1. Lechmann M, et al. 2005. Biochem. Biophys. Res. Commun. 329:132. 2. Kotxor N, et al. 2004. Immunobiology 209:129. 3. Leon F, et al. 2004. J. Immunol. 173:2995. 4. Cramer SO, et al. 2000. Int. Immunol. 12:1347. 5. Fujimoto Y, et al. 2002. Cell 108:755.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD83
mCD83 transfected cells stained with purified Michel-19, followed by anti-rat IgG FITC
Biotin anti-mouse CD83
mCD83 transfected cells stained with biotinylated Michel-19, followed by Sav-PE
FITC anti-mouse CD83
mCD83 transfected cells stained with Michel-19 FITC
PE anti-mouse CD83
mCD83 transfected cells stained with Michel-19 PE
APC anti-mouse CD83
mCD83 transfected cells stained with Michel-19 APC
Alexa Fluor® 488 anti-mouse CD83
mCD83 transfected cells stained with Michel-19 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD83
mCD83 transfected cells stained with Michel-19 Alexa Fluor® 647
Brilliant Violet 650™ anti-mouse CD83
Mouse CD83 transfected cells stained with Michel-19 Brilliant Violet 650™.
