The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation2, in vitro and in vivo blocking2-4 of CTLA-4 Ig to CD80, and immunohistochemical staining1,4 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 104710).
Application References:
1. Harlan, D.M., et al., 1994. PNAS 91:3137. 2. Razi-Wolf, Z., et al., 1992. PNAS 89:4210. 3. Hathcock, K.S., et al., 1994. J. Exp. Med. 180:631. 4. Herold, K.C., et al., 1997. J. Immunol. 158:984. 5. Ma, X-T., et al., 2006. Cancer Research 66:1169. 6. Andoniou, C. E., et al., 2005. Nature Immunology 6: 1011. 7. Lawson, B. R., et al., 2007. J. Immunol. 178:5366. 8. Turnquist, H.R., et al., 2007. J. Immunol. 178:7018. 9. Garidou, L., et al. 2009. J. Virol. 83:8905. PubMed
LPS-stimulated (3 days) BALB/c
splenocytes stained with 16-10A1
FITC
Description:
CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily, also known as B7-1, B7 and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The 16-10A1 antibody has been shown to block the co-stimulation of T cells by activated B cells in vitro and to block the binding of CTLA-4 to CD80.
Other Names:
B7-1, B7, Ly-53
Structure:
Ig superfamily, 60 kD
Distribution:
Macrophages, activated B and T cells, dendritic cells
Function:
T cell costimulation
Ligand Receptor:
CD28 (stimulatory), CD152(CTLA4)(inhibitory)
Antigen References:
1. Barclay, A.N., et al., 1997. The Leukocyte Antigen FactsBook, Academic Press. 2. Linsley, P.S., et al., 1991. J. Exp. Med. 174:561. 3. Salomon B., et al., 2001. Annu. Rev. Immunol. 19:225.
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