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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, and blocking1-3 of CD51 adhesion and LAK cell cytotoxicity. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 104108).
Application References:
1. Takahashi K, et al. 1990. J. Immunol. 145:4371. (IP Block) 2. Narumiya S, et al. 1994. Int. Immunol. 6:139. (Block) 3. Piali L, et al. 1995. J. Cell Biol. 130:451. (Block)
CD51 is a 140 kD protein, also known as αV integrin, vitronectin receptor, and integrin αV. It is a member of the integrin family, expressed on activated T cells, polymorphonuclear granulocytes, platelets, blastocysts, and osteoclasts. CD51 forms heterodimers by association with integrins β1, β3, β5 or β6; these complexes then act as receptors for multiple extracellular matrix proteins (ECM). The αv integrin heterodimers have varied functions in development, stimulation/activation and homeostasis. The primary ligands for CD51 complexes are fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31. The RMV-7 antibody has been reported to block binding of CD51 to vitronectin, fibronectin, and CD31 in some cell types, as well as blocking LAK cell cytotoxicity.
Integrin family, associates with integrins β1, β3, β5 or β6, 140 kD
Distribution:
Activated T cells, polymorphonuclear granulocytes, blastocysts, osteoclasts
Function:
Adhesion
Ligand Receptor:
Fibronectin, fibrinogen, vitronectin, thrombspondin, von Willebrand factor, and CD31
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Maxfield SR, et al. 1989. J. Exp. Med. 169:2173. 3. Piali L, et al. 1995. J. Cell Biol. 130:451.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD51
C57BL/6 bone marrow cells stained
with RMV-7 PE
LEAF™ Purified anti-mouse CD51
C57BL/6 bone marrow cells stained
with RMV-7 PE
PE anti-mouse CD51
C57BL/6 mouse bone marrow cells were stained with CD51 (clone RMV-7) PE (filled histogram) or Rat IgG1, κ PE isotype control (open histogram). Data shown was gated on myeloid cell population.
Con A stimulated C57BL/6 mouse slenocytes were stained with CD3 APC and CD51 (clone RMV-7) PE (above) or Rat IgG1, κ PE isotype control (below).
Purified anti-mouse CD51
C57BL/6 mouse bone marrow cells were stained with CD51 (clone RMV-7) PE (filled histogram) or Rat IgG1, κ PE isotype control (open histogram). Data shown was gated on myeloid cell population.
