Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (in the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 102512).
Application References:
1. Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block) 2. Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block) 3. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block) 4. Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block) 5. Baldwin HS, et al. 1994. Development 120:2539. (IHC) 6. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC) 7. Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
C57BL/6 mouse splenocytes stained with biotinylated MEC13.3, followed by Sav-PE
CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis. The MEC13.3 antibody has been shown to inhibit in vitro and in vivo functions of CD31.
Other Names:
PECAM-1, EndoCAM
Structure:
Ig superfamily, 130-140 kD
Distribution:
Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
Function:
Adhesion
Ligand Receptor:
CD38, αV/β3 integrin
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. DeLisser HM, et al. 1994. Immunol. Today 15:490. 3. Newman PJ, et al. 1990. Science 247:1219.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD31
C57BL/6 splenocytes stained with MEC13.3 APC
Biotin anti-mouse CD31
C57BL/6 mouse splenocytes stained with biotinylated MEC13.3, followed by Sav-PE
FITC anti-mouse CD31
C57BL/6 mouse splenocytes stained with MEC13.3 FITC
LEAF™ Purified anti-mouse CD31
C57BL/6 splenocytes stained with
MEC13.3 FITC
PE anti-mouse CD31
C57BL/6 mouse splenocytes stained with MEC13. 3 PE
Purified anti-mouse CD31
C57BL/6 mouse splenocytes stained with purified MEC13.3, followed by anti-rat IgG FITC
Alexa Fluor® 488 anti-mouse CD31
C57BL/6 mouse splenocytes stained with MEC13.3 Alexa Fluor® 488
Alexa Fluor® 647 anti-mouse CD31
C57BL/6 mouse splenocytes stained with MEC13.3 Alexa Fluor® 647
