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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: in vitro induction of IL-5 production1. For the most successful immunofluorescent staining results, it may be necessary to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 102305/102306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 102304) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 102308).
Application References:
1. Bowen MA, et al. 1996. J. Immunol. 156:442. 2. Punt JA, et al. 1997. J. Exp. Med. 186:1911.
Con A-stimulated (3 days)splenocytes stained with purified mCD30.1, then detected with biotinylated anti-Armenian hamster IgG followed by Sav-PE
CD30 is a 105 kD type I transmembrane glycoprotein, also known as Ber-H2 or Ki-1. It is a member of the tumor necrosis factor receptor (TNFR) superfamily, expressed on activated T, B, and NK cells, and monocytes. CD28, in conjunction with several cytokines, regulates the expression of CD30 on activated T cells. It has been reported that cytokine production and Th1 versus Th2 development is heavily influenced by CD30-CD30 ligand (CD153) interactions.
Other Names:
Ber-H2, Ki-1, TNFRSF8
Structure:
TNFR superfamily, 105-120 kD
Distribution:
Activated T, B and NK cells, monocytes
Function:
Costimulation, regulates negative selection of thymocytes
Ligand Receptor:
CD153 (CD30L)
Antigen References:
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Falini B, et al. 1995. Blood 85:1. 3. Gedrich RW, et al. 1996. J. Biol. Chem. 271:12852.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD30
Con A-stimulated (3 days)splenocytes stained with purified mCD30.1, then detected with biotinylated anti-Armenian hamster IgG followed by Sav-PE
PE anti-mouse CD30
ConA-stimulated C57BL/6 splenocytes (Day 3) stained with mCD30.1 PE
Purified anti-mouse CD30
Con A-stimulated (3 days)splenocytes stained with purified mCD30.1, then detected with biotinylated anti-Armenian hamster IgG followed by Sav-PE
