Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Lee KM., et al. 2006. Blood. Apr 15: 107(8):3181-3188 2. Schatzle JD., et al. 1999. Proc Natl Acad Sci U S A. 96 (7):3870-3875 3. Stepp SE., et al. 1999. Eur J Immunol. 29(8):2392-2399
C57BL/6 splenocytes stained with m2B4 (B6) 458.1 biotin (upper panel) or mouse IgG1 isotype control biotin (lower panel) followed by Sav-PE and anti-mouse CD49b DX5 APC
Description:
Mouse CD244, also known as 2B4, is a receptor belonging CD2 family of proteins involved in non- MHC-restricted cytotoxicity. It is expressed on all natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes, including dendritic epidermal T cells. At least there are two isoforms of CD244.2 proteins that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner, while the short form functions in an activating manner. The ligand of CD244 is CD48, which expressed on all hematopoietic cells. It was reported that CD244 interacts with CD48 is essential for IL-2-driven expansion and activation of murine NK cells. The 2B4 antibody reacts with CD244.2, the 2B4 allloantigen which is expressed on C57BL/6 mice, but not on Balb/c mice.
Other Names:
2B4 B6 Alloantigen
Structure:
A member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF).
Distribution:
All natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes
Function:
Mediate non-MHC-restricted cytotoxicity.
Ligand Receptor:
CD48
Antigen References:
1. Brown MH., et al. 1998. J Exp Med. 188 (11):2083-2090 2. Davis SJ., et al. 1996. Immunol Today. 17 (4):177-187 3. Kumaresan PR., et al. 2000. Immunogenetics. 51(4-5):306-313 4. Latchman Y., et al. 1998. J Immunol. 161 (11) 5809-5812
