Antibodies
|
Search   
Biotin anti-mouse CD19 Antibody
Biotin anti-mouse CD19 Antibody
101504 500 µg $230.00 Qty:
    

Product Details

 
Clone: MB19-1 (See other available formats)
Isotype: Mouse IgA, κ
Reactivity: Mouse
Immunogen: mouse CD19+ pre-B cell line 300.19
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

COA:
Enter Lot#:   
Application
Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation3, and in vitro costimulation (synergistic with anti-IgM) of B cell activation1,2.

Application
References:

  Publication Library
Biotin anti-mouse CD19 Antibody MB19-1 Image 1
C57BL/6 splenocytes stained with MB19-1 biotin, then detected with Sav-PE


Compare all formats



Cell Surface Immunofluorescence Staining Protocol




Description:

CD19 is a 95 kD glycoprotein, also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81 forms a molecular complex integral to B cell activation.

Other Names: B4
Structure: Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution: Pro-B cells to mature B cells (during development), follicular dendritic cells
Function: Modulates B cell activation and differentiation
Ligand Receptor: CD21, CD81, Leu-13
Antigen
References:

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF,et al. 1994. Immunol. Today 15:437.

GeneID: 12478
Latest Publications: View the latest CD19 articles on HighwirePress.com
UniProt: View information about CD19 on UniProt.org
Keywords: Biotin anti-mouse CD19, MB19-1, Biotin, B4, Mouse, Flow Cytometry, Immunology, Antibodies


Product Reviews

  Title Rating Date
- There are currently no reviews for this product. Be the first to review this product to qualify for a $100 Amazon gift card drawing. -
See more reviews using the Reviews Library


Product FAQs

 
How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
For a complete list of FAQs on all our products, visit our FAQ page.
 
Related Products

DescriptionCloneApplications
Biotin anti-mouse CD3ε145-2C11FC, IF, IHC
FITC anti-mouse CD317A2FC
Biotin anti-mouse/human CD45R/B220RA3-6B2FC
FITC anti-mouse IgD11-26c.2aFC
APC StreptavidinFC, ICFC
APC/Cy7 StreptavidinFC, ICFC
Cy5 StreptavidinFC, ICFC
FITC StreptavidinFC, ICFC
PE StreptavidinFC, ICFC
PE/Cy5 StreptavidinFC, ICFC
PE/Cy7 StreptavidinFC, ICFC
Cell Staining BufferFC, ICC, ICFC
TruStain fcX™ (anti-mouse CD16/32)93 FC


Other Formats

DescriptionCloneApplications
FITC anti-mouse CD19MB19-1FC


Related Pathways

Pathway
B cell Development and Activation
B-Cell Receptor Pathway
Innate Immunity


Related Categories

Category
Immunology > Mouse Immunology > Dendritic cells > CD19
Immunology > Mouse Immunology > Stem Cells > CD19
Immunology > Mouse Immunology > B Cells > CD19 > MB19-1
Immunology > Mouse Immunology > CD and Related Molecules > CD19 > MB19-1
Immunology > Mouse Immunology > Costimulatory Molecules > CD19 > MB19-1

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse CD19
    C57BL/6 splenocytes stained with MB19-1

    C57BL/6 splenocytes stained with MB19-1 biotin, then detected with Sav-PE

  • FITC anti-mouse CD19
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with MB19-1 FITC

x
Copyright © 2014 BioLegend, Inc. All rights reserved.