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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation3, and in vitro costimulation (synergistic with anti-IgM) of B cell activation1,2.
CD19 is a 95 kD glycoprotein, also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81 forms a molecular complex integral to B cell activation.
Other Names:
B4
Structure:
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution:
Pro-B to mature B (during development), follicular dendritic cells
Function:
Modulates B cell activation and differentiation
Ligand Receptor:
CD21, CD81, Leu-13
Antigen References:
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF,et al. 1994. Immunol. Today 15:437.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD19
C57BL/6 mouse splenocytes stained with purified MB19-1, followed by anti-mouse IgA FITC
Biotin anti-mouse CD19
C57BL/6 splenocytes stained with MB19-1 biotin, then detected with Sav-PE
FITC anti-mouse CD19
C57BL/6 mouse splenocytes stained with MB19-1 FITC
