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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional reported applications (for the relevant formats) include: immunoprecipitation3, and in vitro costimulation (synergistic with anti-IgM) of B cell activation1,2.
CD19 is a 95 kD glycoprotein, also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81 forms a molecular complex integral to B cell activation.
Ig superfamily, associates with CD21 and CD81, 95 kD
Pro-B cells to mature B cells (during development), follicular dendritic cells
Modulates B cell activation and differentiation
CD21, CD81, Leu-13
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341. 2. Krop I, et al. 1996. Eur. J. Immunol. 26:238. 3. Krop I, et al. 1996. J. Immunol. 157:48. 4. Tedder TF,et al. 1994. Immunol. Today 15:437.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD19
C57BL/6 splenocytes stained with MB19-1 biotin, then detected with Sav-PE
FITC anti-mouse CD19
C57BL/6 mouse splenocytes stained with MB19-1 FITC