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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining1,2 of acetone-fixed frozen sections, and in vitro and in vivo blocking of ligand binding3-5. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106506) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106504) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 45204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 106508).
Application References:
1. Lettesjö H, et al. 2000. J. Immunol. 165:4095. (IHC) 2. Dunn RJ, et al. 1997. J. Histochem. Cytochem. 45:129. (IHC) 3. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550. (Block) 4. Roy M, et al. 1995. Eur. J. Immunol. 25:596. (Block) 5. Foy TM, et al. 1994. J. Exp. Med. 180:157. (Block) 6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
PMA- and ionomycin-stimulated
(18 hrs) BALB/c T cells stained with
MR1 PE
CD154 is a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation. The MR1 antibody has been reported to inhibit the activation of T and B lymphocytes in vitro and antigen-specific lymphocyte responses in vivo.
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Noelle RJ, et al. 1992. P. Natl. Acad. Sci. USA 89:6550. 3. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881. 4. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Biotin anti-mouse CD154
PMA- and ionomycin-stimulated
(18 hrs) BALB/c T cells stained with
MR1 PE
LEAF™ Purified anti-mouse CD154
PMA- and ionomycin-stimulated
(18 hrs) BALB/c T cells stained with
MR1 PE
PE anti-mouse CD154
PMA- and ionomycin-stimulated
(18 hrs) BALB/c T cells stained with
MR1 PE
Purified anti-mouse CD154
PMA- and ionomycin-stimulated
(18 hrs) BALB/c T cells stained with
MR1 PE
