The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Application:
FC
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation, in vitro and in vivo blocking1-4 of ligand binding, and as ELISA capture antibody5. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 106308).
Application References:
1. Walunas, T.L., et al., 1994. Immunity 1:405. 2. Cilio, C.M., et al., 1998. J. Exp. Med. 188:1239. 3. Issazadeh, S., et al., 1999. J. Immunol. 162:761. 4. McCoy, K., et al., 1997. J. Exp. Med. 186:183. 5. Hsu, H-C., et al., 2007. J. Immunol. 178:5357.
Con A-stimulated (day-2) Balb/c mouse splenocytes stained with biotinylated UC10-4B9, followed by Sav-PE
Description:
CD152 is a 33 kD member of the immunoglobulin superfamily also known as CTLA-4 or Ly-56. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance, as well as the development of protective immunity and thymocyte regulation. The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions.
Other Names:
Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56
Structure:
Ig superfamily, 33 kD
Distribution:
Activated T and B cells
Function:
Negative regulator of T cell activation
Ligand Receptor:
CD80 (B7-1), CD86 (B7-2)
Antigen References:
1. Barclay, A., et al., 1997. The Leukocyte Antigen Facts Book, Academic Press. 2. Allison, J.P., et al., 1995. Science 270:932. 3. Waterhouse, P., et al., 1995. Science 270:985. 4. Linsley, P.S., et al., 1991. J. Exp. Med. 174:561.
