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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported (for relevant formats) applications include: Western Blot, blocking function, immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.
Application References:
1. Takakura N, et al. 1996. J. Invest. Dermatol. 107:770.
Mouse fibroblast NIH/3T3 cells are stained with biotinylated APA5, followed by Sav-PE
Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.
Other Names:
PDGF receptor-α, PDGFR-α
Structure:
Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution:
Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.
Function:
Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand Receptor:
PDGFs
Antigen References:
1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551 2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124 3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD140a
Mouse fibroblast cell line NIH/3T3 stained with APA5 PE
C57BL/6 splenocytes stained with APA5 PE
PE anti-mouse CD140a
Mouse fibroblast cell line NIH/3T3 stained with APA5 PE
C57BL/6 splenocytes stained with APA5 PE
APC anti-mouse CD140a
Mouse fibroblast NIH/3T3 cells stained with APA5 APC
Biotin anti-mouse CD140a
Mouse fibroblast NIH/3T3 cells are stained with biotinylated APA5, followed by Sav-PE
