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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Clone OX-86 has been reported to act as an agonist and stimulate OX-40.
Application References:
1. Higgins LM, et al. 1999. J. Immunol. 162:486. (FC, IHC) 2. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695. (Costim)
Con A-stimulated C57BL/6 mouse splenocytes stained with biotinylated OX-86, followed by Sav-PE
CD134 is a type I integral membrane protein also known as OX-40, ACT35, and tumor necrosis factor receptor superfamily member 4 (TNFRSF4). This receptor is expressed on activated CD4+ and CD8+ T cells and B cells. The OX-40 receptor binds to the OX-40 ligand (CD252) to provide a costimulatory signal that is independent of CD28. Blockade of OX40-OX40 ligand interactions has been shown to ameliorate experimental EAE and inflammatory bowel disease, which implies that these interactions are important in the pathogenesis of some autoimmune diseases.
Other Names:
TNFRSF4, ACT35, OX-40
Structure:
TNF receptor superfamily, 50 kD
Distribution:
Activated CD4+ and CD8+ T cells, activated B cells
Function:
Receptor for OX-40 ligand, provides co-stimulatory signal for lymphocyte proliferation independent of CD28. Thought to play a role in the pathogenesis of some autoimmune diseases.
Ligand Receptor:
OX-40 ligand
Antigen References:
1. Al-Shamkhani A, et al. 1996. Eur. J. Immunol. 26:1695. 2. Weinberg AD, et al. 1999. J. Immunol. 162:1818. 3. Akira H, et al. 1999. J. Immunol. 162:7058. 4. Pippig SD, et al. 1999. J. Immunol. 163:6520. 5. Higgins LM, et al. 1999. J. Immunol. 162:486.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD134 (OX-40)
Con A-stimulated C57BL/6 mouse splenocytes stained with purified OX-86, followed by biotinylated anti-rat IgG and Sav-PE
Biotin anti-mouse CD134 (OX-40)
Con A-stimulated C57BL/6 mouse splenocytes stained with biotinylated OX-86, followed by Sav-PE
Brilliant Violet 421™ anti-mouse CD134 (OX-40)
Con A-stimulated (3 days) C57BL/6 splenocytes were stained with CD134 (clone OX-86) Brilliant Violet 421™ (filled histogram) or rat IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
