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Biotin anti-mouse CD11c Antibody
Cat. # Size Price  

117303 50 µg $65.00
    
117304 500 µg $240.00
    
Product Details

Clone: N418 (See other available formats)
Isotype: Armenian Hamster IgG
Isotype Control:Biotin Armenian Hamster IgG Isotype Ctrl
Reactivity: Mouse
Immunogen: Mouse spleen dendritic cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
IF - Validated
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for other applications.

Application
Notes:

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, and immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10).

Application
References:

  Publication Library
 Image 1
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and biotinylated N418 (top) or biotinylated Armenian hamster IgG isotype control (bottom), followed by Sav-PE

 Image 2


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Antigen Details

Description:

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Other Names: αX integrin, integrin αX chain, CR4, p150, ITGAX
Structure: Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution: Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
Function: Cellular adhesion
Ligand Receptor: iC3b, fibrinogen
Antigen
References:
1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.
GeneID: 16411
UniProt: View information about CD11c on UniProt.org
Keywords: Biotin anti-mouse CD11c, N418, Biotin, αX integrin, integrin αX chain, CR4, p150, ITGAX, Mouse, Flow Cytometry, Immunofluorescence microscopy, Immunology, Antibodies
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Related Protocols

Cell Surface Immunofluorescence Staining Protocol
Additional Data

See IF data...
Related Pages

Integrin Explorer
Integrin Explorer Mobile Application
Streptavidin Conjugates

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 3 Revision Date: 2014-10-10
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with N418 APC and PK136 PE

  • Biotin anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and biotinylated N418 (top) or biotinylated Armenian hamster IgG isotype control (bottom), followed by Sav-PE





  • FITC anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and FITC N418 (top) or FITC Armenian hamster IgG isotype control (bottom).





  • PE anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE N418 (top) or PE Armenian hamster IgG isotype control (bottom)





  • Purified anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and purified N418 (top) or purified Armenian hamster IgG isotype control (bottom), followed by anti-Armenian hamster IgG FITC





  • Alexa Fluor® 488 anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and Alexa Fluor® 488 N418 (top) or Alexa Fluor® 488 Armenian hamster IgG isotype control (bottom)





  • Alexa Fluor® 647 anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 647

    Fixed whole mount mouse epidermis

    Fixed whole mount mouse epidermis Langerhans cells were stained with Alexa Fluor® 647 CD11c (red) (clone N418) and PE CD207 (yellow) (clone 4C7). Isotype controls at the same concentrations were used for the negative control (bottom image). Cells were mounted in Prolong Gold and imaged with a Leica SP8 confocal. Image courtesy of Grzegorz Chodaczek and Zbigniew Mikulski at LIAI.





  • PE/Cy5 anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE/Cy5 N418 (top) or PE/Cy5 Armenian hamster IgG isotype control (bottom)





  • PE/Cy7 anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-E (clone M5/114.15.2) and PE/Cy7 N418 (top) or PE/Cy7 Armenian hamster IgG isotype control (bottom)





  • Brilliant Violet 605™ anti-mouse CD11c
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 605™.

  • Alexa Fluor® 700 anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 700 and M5/114.15.2 PE

  • Pacific Blue™ anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with Pacific Blue™ N418

  • APC/Cy7 anti-mouse CD11c
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with I-A/I-E PE and CD11c (clone N418) APC/Cy7 (top) or Armenian hamster IgG APC/Cy7 isotype control (bottom).





  • PerCP/Cy5.5 anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with N418 PerCP/Cy5.5

  • PerCP anti-mouse CD11c
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with N418 PerCP and M5/114.15.2 PE

  • Brilliant Violet 421™ anti-mouse CD11c
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 421™ (top) or Armenian hamster IgG Brilliant Violet 421™ isotype control (bottom).





    View details and larger image

    View details and larger image by clicking on More Data below...

  • Brilliant Violet 570™ anti-mouse CD11c
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 570™ (top) or Armenian hamster IgG Brilliant Violet 570™ isotype control (bottom).





  • Brilliant Violet 785™ anti-mouse CD11c
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 785™.

  • Brilliant Violet 510™ anti-mouse CD11c
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with mouse I-A/I-E APC and CD11c (clone N418) Brilliant Violet 510™ (top) or Armenian hamster IgG Brilliant Violet 510™ isotype control (bottom).





  • Brilliant Violet 650™ anti-mouse CD11c
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with mouse I-A/I-E PE and CD11c (clone N418) Brilliant Violet 650™ (top) or Armenian hamster IgG Brilliant Violet 650™ isotype control (bottom).





  • Purified anti-mouse CD11c (MaxPar® Ready)
    Mouse splenocytes stained with 147Sm-anti-CD45

    Mouse splenocytes stained with 147Sm-anti-CD45 (30-F11) and 142Nd-anti-CD11c (N418). Data provided by DVS Sciences.

  • Alexa Fluor® 594 anti-mouse CD11c
    C57BL/6 mouse frozen spleen section

    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 5 µg/ml of CD11c (clone N418) Alexa Fluor® 594 (red), 2.5 µg/ml of CD3 (clone 145-2C11) Alexa Fluor® 647 (green), and 2.5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C. The image was captured by 10X objective.

  • PE/Dazzle™ 594 anti-mouse CD11c
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and CD11c (clone N418) PE/Dazzle™ 594.

  • Brilliant Violet 711™ anti-mouse CD11c
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with mouse I-A/I-E PerCP/Cy5.5 and CD11c (clone N418) Brilliant Violet 711™.

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