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The CD11b antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The CD11b antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this CD11b antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8,immunofluorescence microscopy6,7,10 and immunohistochemistry of acetone-fixed frozen sections5,11-13 and paraffin sections28.
Application References:
1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP) 2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete) 3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block) 4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP) 5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC) 6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF) 7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF) 8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete) 9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block) 10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF) 11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC) 12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC) 13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC) 14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC) 15. Olver S, et al. 2006. Cancer Research 66:571. (FC) 16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed 17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC) 18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) 19. Fazilleau N, et al. 2007. Nature Immunol. 8:753. 20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed 21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed 22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed 23. Kim DD, et al. 2008. Blood 112:1109. PubMed 24. Guo Y, et al. 2008. Blood 112:480. PubMed 25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed 26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed 27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed 28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
C57BL/6 mouse bone marrow cells were stained with biotinylated CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by Sav-PE (gated on total cells).
CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.
Other Names:
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR
Structure:
Integrin family, associates with integrin ß2 (CD18), 170 kD
Distribution:
Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram) (gated on total cells).
Biotin anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with biotinylated CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by Sav-PE (gated on total cells).
FITC anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) FITC (top) or rat IgG2b, κ FITC isotype control (bottom) (gated on total viable cells).
LEAF™ Purified anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with LEAF™ purified CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC (gated on total cells).
PE anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram) (gated on total cells).
PE/Cy5 anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells stained with CD11b (clone M1/70) PE/Cy5 (filled histogram) or rat IgG2b, κ PE/Cy5 isotype control (open histogram) (gated on total cells).
Purified anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with purified CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC (gated on total cells).
PE/Cy7 anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PE/Cy7 (filled histogram) or rat IgG2b, κ PE/Cy7 isotype control (open histogram) (gated on total cells).
Alexa Fluor® 488 anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 488 (filled histogram) or rat IgG2b Alexa Fluor® 488 isotype control (open histogram) (gated on total cell population).
Alexa Fluor® 647 anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 647 or rat IgG2b, κ Alexa Fluor® 647 isotype control (gated on total cells).
Alexa Fluor® 700 anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 700 (filled histogram) or rat IgG2b, κ Alexa Fluor® 700 isotype control (open histogram) (gated on total cells).
Pacific Blue™ anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram) (gated on total cells).
APC/Cy7 anti-mouse/human CD11b
C57BL/6 mouse bone marrow stained with M1/70 APC/Cy7
PerCP/Cy5.5 anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PerCP/Cy5.5 (filled histogram) or rat IgG2b, κ PerCP/Cy5.5 isotype control (open histogram) (gated on total cells).
PerCP anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) PerCP (filled histogram) or rat IgG2b PerCP isotype control (open histogram) (gated on total cell population).
Brilliant Violet 421™ anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.
Brilliant Violet 570™ anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 570™ (filled histogram) or rat IgG2b, κ Brilliant Violet 570™ isotype control (open histogram). Data shown was gated on myeloid cell population.
Brilliant Violet 605™ anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 605™. Data shown was gated on myeloid cell population.
Brilliant Violet 650™ anti-mouse/human CD11b
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 650™. Data shown was gated on myeloid cell population.
