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The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration:
0.5 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemical staining1,2 of acetone-fixed frozen sections, and in vitro activation1.
CD103 is a type I transmembrane glycoprotein known as αE integrin or Integrin αIEL chain. It belongs to integrin family and is primarily found on intestinal intraepithelial lymphocytes (IEL). CD103 is also expressed on a subpopulation of lamina propria T cells, epithelial dendritic cells, lamina propria-derived dendritic cells, and a small subset of peripheral lymphocytes. T regulatory cells express high level of CD103. The CD103 expression on lymphocytes can be induced upon activation and TGF-β stimulation. In association with integrin β7, CD103 is expressed as αE/β7 heterodimer. Mature CD103 protein can be cleaved into 2 chains, a 150 kD (C-terminal) chain and a 25 kD (N-terminal) chain, which remain linked by disulfide bonds. CD103 binds to E-cadherin and mediates homing of lymphocytes to the intestinal epithelium.
Type I transmembrane glycoprotein, Integrin family, can be cleaved into 150 kD and 25 kD chains, associated with β7 integrin
Distribution:
Majority of intestinal intraepithelial lymphocytes (IEL), subpopulation of lamina propria T cells, epithelial dendritic cells, small subset of peripheral lymphocytes, Treg cells.
Function:
Retention and activation of CD103+ lymphocytes in the intestinal epithelium, regulate tissue-specific T cell homing.
Ligand Receptor:
E-Cadherin
Antigen References:
1. Kilshaw PJ and SJ. Murant. 1990. Eur. J. Immunol. 20:2201. 2. Karecla PI, et al. 1995. Eur. J. Immunol. 25:852. 3. LeFrancois L, et al. 1994. Eur. J. Immunol. 24:635. 4. Sung SS, et al. 2006. J. Immunol. 176:2161. 5. Johansson-Lindbom B, et al. 2005. J. Exp. Med. 202:1063. 6. Dujardin HC, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:14473.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD103
C57BL/6 mouse splenocytes stained with PE CD3 (clone 17A2) and purified 2E7, followed by anti-Armenian hamster IgG FITC
Biotin anti-mouse CD103
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and biotinylated 2E7, followed by Sav-PE
PE anti-mouse CD103
C57BL/6 splenocytes stained with 2E7 PE
Alexa Fluor® 488 anti-mouse CD103
C57BL/6 mouse splenocytes stained with PE CD3 (clone 17A2) and Alexa Fluor® 488 2E7
Alexa Fluor® 647 anti-mouse CD103
C57BL/6 mouse splenocytes stained with PE CD3 (clone 17A2) and Alexa Fluor® 647 2E7
APC anti-mouse CD103
BALB/c splenocytes stained with 2E7 APC
PerCP/Cy5.5 anti-mouse CD103
C57BL/6 splenocytes stained with CD3 (145-2C11) APC and 2E7 PerCP/Cy5.5
Pacific Blue™ anti-mouse CD103
C57BL/6 splenocytes stained with 2E7 Pacific Blue™ and CD3e (145-2C11) PE
C57BL/6 splenocytes stained with Armenian Hamster isotype control Pacific Blue™ and CD3e (145-2C11) PE
FITC anti-mouse CD103
C57BL/6 mouse splenocytes were stained with CD3 APC and CD103 (clone 2E7) FITC (top) or Armenian hamster IgG FITC isotype control (bottom).
Brilliant Violet 421™ anti-mouse CD103
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD103 (clone 2E7) Brilliant Violet 421™ (top), or Armenian hamster IgG Brilliant Violet 421™ isotype control (bottom).
